本研究成功利用高分子薄膜材料開發出血漿血球分離晶片，並結合微型幫浦來發展出快速血漿血球分離系統平台。研究方向是利用濾餅過濾(Dead-end filtration)來分離全血中的血球細胞，傳統的濾餅過濾是以壓力為驅動力，懸浮液流動方向與過濾薄膜表面垂直，懸浮液直接壓向薄膜面，通過薄膜而得到濾液，適合短時間操作以及小量批次處理。本實驗利用微機電製程技術(Micro electro mechanical systems, MEMS)製作出聚甲基丙烯酸甲酯(Polymethylmethacrylate, PMMA)分離晶片，最後結合高分子薄膜材料與PMMA分離晶片，即完成血漿血球分離晶片，再透過以頻率控制流量的微型幫浦來驅動血液過濾。本研究將對不同過濾薄膜面積(56.25 mm2、100 mm2及225 mm2)、不同血液檢體量(60 μL、80 μL、100 μL、120 μL、140 μL、160 μL及180 μL)及不同驅動頻率(10 Hz、20 Hz、30 Hz、40 Hz、50 Hz及60 Hz)去進行血漿血球分離的探討。由實驗結果發現，在過濾薄膜面積225 mm2、血液檢體量140 μL及驅動頻率40 Hz到60 Hz間有血漿收集率最佳可達到58%以上；在驅動頻率為10 Hz到50 Hz下對於血球產生較小的溶血現象(溶血率0.33%~0.38%)，所以過濾面積、血液檢體量及驅動頻率對於血漿收集有影響性。最後，本血漿血球分離晶片透過雷射雕刻技術以及雙面黏性材料來完成可拋棄式血漿血球分離晶片，晶片尺寸為30 mm× 20 mm× 5 mm，結合可時間控制的微型幫浦，開發出快速血漿血球分離系統平台。本研究所開發出的快速血漿血球分離系統平台有別於一般傳統離心機，用於分離血球與血漿有著良好的過濾效果與血漿收集率，可在10分鐘內完成過濾，並具備不易溶血(溶血率<0.5%)、可攜帶、低成本(<6元/個)及容易操作等優點。

This study successfully develops the macromolecule membrane filtration chip to separate blood cells and plasma, and develops system which combine micropump with filtration chip. The filtration chip is based on dead-end filtration to separate blood cells in whole blood. The dead-end filtration used pressure as force to make the fluidic pass through the membrane and get the filtrate. The filtration chip uses the laser ablation of MEMS process to manufacture polymethylmethacrylate (PMMA) filtration chip. Then, combined macromolecule membrane with PMMA chip to complete whole blood filtration chip. This study was discussed the plasma collection under different membrane sizes(56.25 mm2, 100 mm2 and 225 mm2), different volume of whole blood(60 μL, 80 μL, 100 μL, 120 μL, 140 μL, 160 μL and 180 μL) and different frequencies(10 Hz, 20 Hz, 30 Hz, 40 Hz, 50 Hz and 60 Hz). When the membrane size is 225 mm2, volume of whole blood is 140 μL and frequencies are between 40 Hz and 60 Hz, the plasma collection rate is up to 58%. The frequency between 10 Hz and 50 Hz have small hemolysis(hemolysis rate is between 0.33% and 0.38%). Finally, this study develops the rapid separation system which combined time-controlled micropump and disposable filtration chip was made by laser ablation and double-side material, the chip size is 30 mm× 20 mm× 5 mm. This study has better effect on blood cells and plasma separation than other experiment results, and it can rapidly finish separation in 10 minutes, and avoid hemolysis.

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