近年來貴金屬(如金、鈀、鉑與銠)在目前全世界的需求與市場顯著增加，貴金屬本身具有特殊的物理與化學特性，導致貴金屬礦業製程處理需要昂貴技術，因此造就了貴金屬之高價位。目前在眾多回收貴金屬方法中，有一種低處理成本且高選擇性的方法，即利用微生物或是微生物產物-蛋白質進行貴金屬之吸附。文獻顯示一些蛋白質表面上所帶的官能基可提供特別的金屬鍵結中心(金屬鍵結位置)來進行金屬離子之螯合。本研究企圖利用自環境分離純化出的嗜熱菌株(Thermophilic strain) 所生產的蛋白質，開發生物吸附技術進行貴金屬(如金、鈀、鉑與銠)之回收。結果顯示，培養基以Modified Thermus Enhanced為最適合進行培養該嗜熱菌株生產金屬鍵結蛋白質，其最適化培養條件分別為溫度控制為55oC，攪拌速率控制為200 rpm，初始酸鹼值為7.0，最適合碳源為蔗糖，蔗糖濃度為0.5 g/L，尿素為最佳氮源，碳氮比為2.0為最佳組成。接著以該嗜熱菌株所生產蛋白質分別探討金、鈀、鉑與銠的吸附效能，結果顯示，鉑的最佳吸附條件的酸鹼值為2，吸附效能為231.6 mg/g protein；金的最佳吸附條件的酸鹼值為1，吸附效能為482.0 mg/g protein；鈀的最佳吸附條件的酸鹼值為3，吸附效能為395.5 mg/g protein；銠的最佳吸附條件的酸鹼值為3，吸附效能為712.7 mg/g protein。當吸附條件中藉由將溫度的提升，其對吸附效能也會有提升的效用。當吸附條件的溫度調升至100oC時，貴金屬吸附效能分別為252.2 mg Pt/mg protein、483.2 mg Au/mg protein、727.4 mg Pd/mg protein、386.0 mg Rh/mg protein。等溫吸附曲線於室溫下進行操作，貴金屬的飽和吸附曲線可以Freundlich等溫方程式描述。最後在脫附劑的測試中，以1.5 M的Thiourea結合1.0 N HCl可以有效地將貴金屬於生物蛋白上脫附，使貴金屬回收效能可達90%以上。

The global demands and market prices of noble metals (e.g., Aurum (or gold), Platinum and Rhodium) have markedly increased. Because of special physical and chemical characteristics of noble metals, the process of noble metal mining requires employment of expensive technology, making the cost of mining process higher than received economic benefits. One of the ways to collect rare earth metal with low mining process cost and high selectivity is using microorganisms or their derived products (such as proteins) to directly adsorb the metals. Literature shows that some proteins possess functional groups that can provide specific metal binding sites (metal-binding domains), to chelate metal ions. In this study, noble metals, such as Aurum (or gold), Rhodium, and Platinum in aqueous system were recovered via biosorption using proteins produced from an isolated bacterial strain Thermophilic strain. Modified Thermus Enhanced Medium was found to be a suitable medium to improve the protein production of the thermophilic strain. The optimal conditions of protein production were temperature, 55oC; agitation rate, 200 rpm; initial pH, 7. Sucrose with a concentration of 0.5 g/L was selected as carbon source and urea as nitrogen source. A carbon to nitrogen ratio (C/N ratio) of 2 was considered as the best composition of carbon and nitrogen concentration to increase the protein production. Finally the microbial protein produced from thermophilic strain showed effective adsorption ability for Platinum (Pt), Aurum (Au), and Rhodium (Rh). The optimum adsorption condition for Pt occurred at pH 2 with an adsorption capacity of 231.6 mg/g protein. For Au, the optimum adsorption condition occurred at pH 1 with an adsorption capacity of 482.0 mg/g protein. For Pd metal, the optimum adsorption condition occurred at pH 3 with an adsorption capacity of 395.5 mg/g protein. For Rh metal, the adsorption optimum condition occurred at pH 3 with an adsorption capacity of 712.7 mg/g protein. The metal adsorption capacity increased with an increase in temperature. When the temperature was increased to 100oC, the metal adsorption capacity for Pt, Au, Pd, and Rh reached 252.2, 483.2, 727.4, and 386.0 mg/mg protein. The adsorption isotherm was conducted at room temperature. The adsorption of Pt, Au, Pd, and Rh could be well fitted by the Freundlich isotherm equation. Among the desorption agents examined, combination of 1.5 M Thiourea with 1 N HCl can effectively desorb the Pt, Au, Pd, and Rh metal ion from microbial protein with over 90% metal recovery.

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