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研究生: 游琍芽
Farahdiba, Aulia Ulfah
論文名稱: 混凝沈澱法和溶解氣體浮除程序對水中藍綠藻的去除研究
Removal of Cyanobacteria Cells using Coagulation and Dissolved Air Flotation Process
指導教授: 林財富
LIN, TSAIR FUH
學位類別: 碩士
Master
系所名稱: 工學院 - 環境工程學系
Department of Environmental Engineering
論文出版年: 2014
畢業學年度: 102
語文別: 英文
論文頁數: 108
外文關鍵詞: coagulation/flocculation, Cylindropsermopsis raciborskii, dissolved air flotation, Microcystis aeruginosa, sedimentation
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  • Dissolved air flotation (DAF) is a process known to efficiently remove high concentrations of cyanobacteria under low turbidity. In applying DAF, coagulation and flocculation (C/F) are commonly used together to improve the processes. However, the performance is often dependent on cyanobacteria species, and coagulant types and doses. In this study, C/F process was combined with sedimentation (S) and DAF and two types of coagulants, aluminum sulfate (AS) and ferric chloride (FC), to examine the removal of two cyanobacteria species, a unicellular Microcystis aeruginosa and a filamentous Cylindropsermopsis raciborskii.

    Results showed that performance of C/F/S and C/F/DAF processes relies on coagulation pH, coagulant type, and coagulant dose. Removal efficiency of M. aeruginosa and C. circinallis cells were very distinct. The optimum dosages for M. aeruginosa cell removal with C/F/S were 40 mg/L for AS and 30 mg/L for FC, with removal efficiency of 96.5-98.0 %. C/F/DAF outperformed C/F/S in M. aeruginosa cell removal. With 30 mg/L AS and FC as optimum doses, 97.3-99.2% of removal can be achieved. When treating C. raciborskii with C/F/S, removal efficiencies were low, only 10-20% and 20-30% for the cases of using AS and FC as coagulants, respectively. Higher removal efficiencies of C. raciborskii cells were observed when using C/F/DAF, with 70-80% for using FC and 50-60% for AS. C/F/S and C/F/DAF were proved to effectively remove unicellular M. aeruginosa cells, but not filamentous C. raciborskii cells. During treatment, the two processes remove the whole cells, and no significant amount of intracellular toxins were released into the water.

    ABSTRACT I ACKNOWLEDGEMENT III TABLE OF CONTENTS V LIST OF TABLE IX LIST OF FIGURE X CHAPTER 1 INTRODUCTION 1 1.1 Background 1 1.2 Objectives of study 2 CHAPTER 2 LITERATURE REVIEW 3 2.1 Blue-Green Algae (Cyanobacteria) 3 2.1.1 Size Range and Diversity of Structure of algae/cyanobacteria 3 2.1.2 Gas vacuoles 9 2.1.3 Microcystis aeruginosa 11 2.1.4 Cylindrospermopsis raciborskii 12 2.1.5 Cyanotoxins and Algal Metabolites 13 2.2 Coagulation-Flocculation 15 2.2.1 Surface charge and electrical double Layer 18 2.2.2 Aluminum Sulfate (Al2(SO4)3.14H20) 20 2.2.3 Ferric Chloride (FeCl3) 22 2.2.4 Dissolved Air Flotation (DAF) 25 2.2.4.1 General 25 2.2.4.2 Operating conditions 27 2.3 Algae removal with DAF 28 CHAPTER 3 METHODOLOGY 29 3.1 The approach of this work 29 3.2 Water Matrix 30 3.3 Material and methods 32 3.3.1 Culture Algae 32 3.3.2 Sample preparation 32 3.3.3 Cell enumeration 32 3.3.3.1 Microcystis aeruginosa 33 3.3.3.2 Cylindrospermopsis raciborrskii 33 3.3.4 Measurement of cyanotoxins 34 3.3.5 Preparation of Coagulant 34 3.3.6 Zeta potential 34 3.3.7 Flocs-Bubble Aggregates 35 3.3.8 Turbidity and pH 35 3.3.9 Nutrient 35 3.4 Coagulation-Flocculation-Sedimentation (C/F/S) Experiments 35 3.5 Dissolved Air Flotation (DAF) Experiments 36 CHAPTER 4 RESULTS AND DISCUSSIONS 38 4.1 Zeta Potential (Kinmen’s Water) 38 4.2 Removal of Microcystis aeruginosa (M.aeruginosa) cells 39 4.2.1 Effects on pH 39 4.2.2 Zeta Potential 43 4.2.3 Coagulation-Flocculation-Sedimentation (C/F/S) 45 4.2.3.1 Turbidity 45 4.2.3.2 Removal of M. aeruginosa cells 47 4.2.4 Coagulation-Flocculation-Dissolved Air Flotation (C/F/DAF) 49 4.2.4.1 Turbidity 49 4.2.4.2 Removal of M. aeruginosa 51 4.2.5 Comparison between C/F/S and C/F/DAF 53 4.2.5.1 Turbidity 53 4.2.5.2 Removal M. aeruginosa 55 4.2.6 MC-LR removal by C/F/S and C/F/DAF 58 4.3 Removal of Cylindrospermopsis raciborskii (C. raciborskii) cells 62 4.3.1 Effects on pH 62 4.3.2 Zeta Potential 65 4.3.3 Coagulation- Flocculation-Sedimentation (C/F/S) 67 4.3.3.1 Turbidity 67 4.3.3.2 C.raciborskii removal 69 4.3.4 Coagulation-Flocculation-Dissolved Air Flotation (C/F/DAF) 71 4.3.4.1 Turbidity 71 4.3.4.2 Removal of C. raciborskii cells 72 4.3.5 Comparison between C/F/S and C/F/DAF 74 4.3.5.1 Turbidity 74 4.3.5.2 Removal of C. raciborskii cells 75 4.3.6 Cylindrospermopsins (CYN) removal by C/F/S and C/F/DAF 78 4.4 Comparison between M. aeruginosa and C. raciborskii by DAF - C/F/DAF 81 4.5 Floc-Bubble Aggregates 83 4.6 Summaries of cyanobacteria cell removal by C/F/S and C/F/DAF 89 CHAPTER 5 CONCLUSIONS AND RECOMMENDATIONS 91 5.1 Conclusions 91 5.2 Recommendations 92 REFERENCES 93 APPENDIX A - Algae Medium Culture 102 APPENDIX B - Algae Species 104 APPENDIX C - Picture 106 APPENDIX D - Calibration Curves 108

    Aktas, T. S., F. Takeda, C. Maruo, N. Chiba and O. Nishimura (2012). "A comparison of zeta potentials and coagulation behaviors of cyanobacteria and algae." Desalination and Water Treatment 48(1-3): 294-301.
    Amirtharajah, A. and K. J. Mills (1982). "Rapid-mix design for mechanisms of alum coagulation." J Am Water Works Assoc 74(4): 210-216.
    Aparecida Pera do Amaral, P., Coral, Lucila Adriani., Nagel-Hassemer, Maria Eliza., Belli, Tiago José., Lapolli, Flávio Rubens (2013). "Association of dissolved air flotation (DAF) with microfiltration for cyanobacterial removal in water supply." Desalination and Water Treatment 51(7-9): 1664-1671.
    APHA (1992). Standard Methods for the Examination of Water and Wastewater. A. P. H. Association. Washington, DC
    Bellinger, E. G. and D. C. Sigee (2010). Freshwater Algae. United Kingdom, John Wiley & Sons.
    Bernhardt, H. and J. Clasen (1994). "Investigations into the flocculation mechanisms of small algal cells." AQUA 43(5): 222-232.
    Bickerton, B. J. (2012). Optimization of Dissolved Air Flotation for Drinking Water Treatment. Master of Applied Science, Dalhousie University.
    Bitton, G. (2005). Wastewater Microbiology. Canada, John Wiley & Sons.
    Bratby, J. (2006). Coagulation and flocculation in water and wastewater treatment. London, UK, IWA Publishing.
    Carmichael, W. (1994). "The toxins of cyanobacteria." Scientific American 270(1): 78-86.
    Carr, N. G. and B. A. Whitton (1982). The Biology of Cyanobacteria. Berkeley and Los Angeles, University of California Press.
    Chen, J. J., H. H. Yeh and I. C. Tseng (2008). "Potassium permanganate as an alternative preoxidant for enhancing algal coagulation--pilot and bench scale studies." Environ Technol 29(7): 721-729.
    Chen, J. J., H. H. Yeh and I. C. Tseng (2009). "Effect of ozone and permanganate on algae coagulation removal--pilot and bench scale tests." Chemosphere 74(6): 840-846.
    Chow, C. W. K., J. House, R. M. A. Velzeboer, M. Drikas and M. D. Burch (1999). "The impact of conventional water treatment processes on cells of the cyanobacterium Microcystis aeruginosa." Water Res 33(15): 3253-3262.
    Chow, C. W. K., J. House, R. M. A. Velzeboer, M. Drikas, M. D. Burch and D. A. Stefensen (1998). "The effect of ferric chloride flocculation on cyanobacterial cells." Water Res 32(3): 808-814.
    Cvijan, M. and S. Fujinato (2012). "Cylindrospermopsis raciborskii (Cyanoprokaryota) –
    potential invasive and toxic species in Serbia." Botanica SERBICA 36(1): 3-8.
    Dixon, M. B., Y. Richard, L. Ho, C. W. K. Chow, B. K. O'Neill and G. Newcombe (2011). "A coagulation-powdered activated carbon-ultrafiltration--multiple barrier approach for removing toxins from two Australian cyanobacterial blooms." J Hazard Mater 186(2-3): 1553-1559.
    Duan, J. and J. Gregory (2003). "Coagulation by hydrolysing metal salts." Advances in Colloid and Interface Science 100-102: 475-502.
    Eddy, M. a. (2003). Wastewater Engineering: Treatment and Reuse, McGraw-Hill Education.
    Edzwald, J. K. (1990). "Chemical and Physical aspects of dissolved-air flotation for the removal of algae." AQUA 39: 24-35.
    Edzwald, J. K. (1995). "Principles and applications DAF." Water Science Technology 31(3-4): 1-23.
    Edzwald, J. K. (2010). "Dissolved air flotation and me." Water Res 44(7): 2077-2106.
    Edzwald, J. K. and J. Haarhoff (2012). Dissolved Air Flotation For Water Clarification. New York, McGraw-Hill.
    Edzwald, J. K., J. P. Walsh, G. S. Kamiski and H. Dunn (1992). "Flocculation and air requirements for dissolved air flotation." American Water Works Association 84(3): 92-100.
    Eikelboom, D. H. (1975). "Filamentous organism observed in activated sludge." Water Res
    9: 365-388.
    El-Gohary, F., A. Tawfik and U. Mahmoud (2010). "Comparative study between chemical coagulation/precipitation (C/P) versus coagulation/dissolved air flotation (C/DAF) for pre-treatment of personal care products (PCPs) wastewater." Desalination 252(1-3): 106-112.
    Falconer (2005). Cyanobacterial toxins of drinking water supplies: cylindrospermopsins and microcystins. CRC Boca Raton.
    Ferguson, C., G. S. Logsdon and D. Curley (1995). "Comparison of dissolved air flotation and direct filtration." Water Science and Technology 31(3): 113-124.
    Gao, S., J. Yang, J. Tian, F. Ma, G. Tu and M. Du (2010). "Electro-coagulation-flotation process for algae removal." J Hazard Mater 177(1-3): 336-343.
    Ghernaout, B., D. Ghernaout and A. Saiba (2010). "Algae and cyanotoxins removal by coagulation/flocculation: A review." Desalination and Water Treatment 20(1-3): 133-143.
    Gregory, J. (2006). Particles in Water: Properties and Processes. London, IWA Publishing.
    Gregory, R. and T. F. Zabel (1990 ). Water Quality and Treatment, A Handbook of Community Water Supplies. New York, McGraw Hill.
    Han, M. and S. Dockko (1998). "Zeta Potential Measurement of Bubbles in DAF Process and Its Effect on the Removal Efficiency." Environmental Engineering 2(4): 461-466.
    Hargesheimer, E. E., Watson, Susan B. (1996). "Drinking water treatment options for taste and odor control." Water Res 30(6): 1423-1430.
    Henderson, R., M. Chips, N. Cornwell, P. Hitchins, B. Holden, S. Hurley, S. A. Parsons, A. Wetherill and B. Jefferson (2008). "Experiences of algae in UK waters: a treatment perspective." Water and Environment Journal 22(3): 184-192.
    Henderson, R., S. A. Parsons and B. Jefferson (2008). "The impact of algal properties and pre-oxidation on solid-liquid separation of algae." Water Res 42(8-9): 1827-1845.
    Henderson, R. K., S. A. Parsons and B. Jefferson (2008). "Successful Removal of Algae through the Control of Zeta Potential." Separation Science and Technology 43(7): 1653-1666.
    Henderson, R. K., S. A. Parsons and B. Jefferson (2010). "The impact of differing cell and algogenic organic matter (AOM) characteristics on the coagulation and flotation of algae." Water Res 44(12): 3617-3624.
    Ho, L., J. Dreyfus, J. Boyer, T. Lowe, H. Bustamante, P. Duker, T. Meli and G. Newcombe (2012). "Fate of cyanobacteria and their metabolites during water treatment sludge management processes." Sci Total Environ 424: 232-238.
    Hsieh, W.-H., D.-W. Chang and T.-F. Lin (2013). "Occurrence and Removal of Earthy-Musty Odorants in Two Waterworks in Kinmen Island, Taiwan." Journal of Hazardous, Toxic, and Radioactive Waste: 130709224445004.
    Huang, C.-F. and H.-H. Yeh (2009). "Study on algae removal." water science amd Technology: Water Supply 9(2): 167–172.
    Jones, W. W. and S. Sauter (2005). Cylindrospermopsis raciborskii in Indiana Lakes and Reservoirs, Indiana University.
    Kempeneers, S., F. Van Menxel and L. Gille (2001). "A decade of large scale experience in dissolved air flotation." Water Science & Technology 43(8): 27-34.
    Kitchener, J. A. and R. J. Gochin (1981). "The mechanism of dissolved air flotation for potable water basic analysis and a proposal." Water Res 15: 585-590.
    Li, J., B. Hu, P. Zheng, M. Qaisar and L. Mei (2008). "Filamentous granular sludge bulking in a laboratory scale UASB reactor." Bioresour Technol 99(9): 3431-3438.
    Lund, H. C. and J. W. Lund (1995). Freshwater Algae - Their microscopic world explored. England, Biopress Ltd.
    Ma, J., G. Lei and J. Fang (2007). "Effect of algae species population structure on their removal by coagulation and filtration processes- a case study." Water Supply 56(1).
    Malley, J. P. and J. K. Edzwald (1991). "Laboratory comparison of DAF with conventional treatment." American Water Works Association 83(9): 56–61.
    Marbun, Y. R., H.-K. Yen, T.-F. Lin, H.-L. Lin and A. Michinaka (2012). "Rapid on-site monitoring of cylindrospermopsin-producers in reservoirs using quantitative PCR." Sustainable Environmental Research 22(3): 143-151.
    Markham, L., M. Porter and T. Schofield (1997). Algae and Zooplankton Removal by Dissolved Air Flotation at Severn Trent Ltd. SurfaceWater TreatmentWorks. CIWEM Dissolved Air Flotation International Conference, London, UK, The Lavenham Press, London.
    Mauclaire, L., A. Schürmann and F. Mermillod-Blondin (2006). "Influence of hydraulic conductivity on communities of microorganisms and invertebrates in porous media: a case study in drinking water slow sand filters." Aquatic Sciences 68(1): 100-108.
    Morrison, S., A. Venter and S. Barnard (2012). "A case study to determine the efficacy of ozonation in purification processes." Water SA 38(1).
    Mouchet, P. and V. Bonnelye (1998). "Solving algae problems: Prench expertise and world-wide applications." AQUA 27(1998): 125-141.
    Plummer, J. D. and J. K. Edzwald (2002). "Effects of chlorine and ozone on algal cell properties and removal of algae by coagulation." Water supply Research and technology 51(6): 207-218.
    Ralston, J., D. Fornasiero and R. Hayes (1999). "Bubble–particle attachment and detachment in flotation." Int. J. Miner. Process 56: 133-164.
    Reynolds, C. S., R. L. Oliver and A. E. Walsby (1987). "Cyanobacterial dominance: The role of buoyancy regulation in dynamic lake environments." New Zealand Journal of Marine and Freshwater Research 21(3): 379-390.
    Rippka, R. (1988). "Isolation and Purification of Cyanobacteria." Method Enzymol 167: 3-27.
    Roh, S. H., D. H. Kwak, H. J. Jung, K. J. Hwang, I. H. Baek, Y. N. Chun, S. I. Kim and J. W. Lee (2008). "Simultaneous Removal of Algae and Their Secondary Algal Metabolites from Water by Hybrid System of DAF and PAC Adsorption." Separation Science and Technology 43(1): 113-131.
    Schmidt, W., H. Willmitzer, K. Bornmann and J. Pietsch (2002). "Production of drinking water from raw water containing cyanobacteria--pilot plant studies for assessing the risk of microcystin breakthrough." Environ Toxicol 17(4): 375-385.
    Scholfield, T. (2001). "Dissolved air flotation in drinking water production." Water Science Technology 43(8): 9-18.
    Sigee, D. C. (2005). Freshwater Microbiology. England, John Wiley & Sons.
    Steffensen, D. A. and B. C. Nicholson (1994). Toxic Cyanobacteria -Current Status of Research and Management. Proceedings of an International Workshop, Adelaide, Australia, American Water Works Association Research Foundation, Australian Centre for Water Quality Research, Centre for Water Research, , Belgium.
    Stumm, W. and J. J. Morgan (1981). Aquatic chemistry : an introduction emphasizing chemical equilibria in natural waters. New York, Wiley.
    Sun, F., H.-Y. Pei, W.-R. Hu and C.-X. Ma (2012). "The lysis of Microcystis aeruginosa in AlCl3 coagulation and sedimentation processes." Chemical Engineering Journal 193-194: 196-202.
    Taki, K., T. Seki, S. Mononobe and K. Kato (2008). "Zeta potential measurement on the surface of blue-green algae particles for micro-bubble process." Water Sci Technol 57(1): 19-25.
    Teixeira, M. and M. Rosa (2007). "Comparing dissolved air flotation and conventional sedimentation to remove cyanobacterial cells of Microcystis aeruginosaPart II. The effect of water background organics." Separation and Purification Technology 53(1): 126-134.
    Teixeira, M. R. (2005). Development of flotation and nanofiltration technologies to remove cyanobacteria and cyanotoxins in drinking water treatment. Doctor, University of Algarve.
    Teixeira, M. R. and M. J. Rosa (2006). "Comparing dissolved air flotation and conventional sedimentation to remove cyanobacterial cells of Microcystis aeruginosa." Separation and Purification Technology 52(1): 84-94.
    Tenney, M. W., Stumm, Werner (1965). "Chemical Flocculation of Microorganisms in Biological Waste Treatment." Water Pollution Control Federation 37(10): 1370-1388.
    Udeh, P. J. (2004). A guide to Healthy Drinking Water. United States, iUniverse.
    Vlaski, A. (1998). Microcystis aeruginosa Removal by Dissolved Air Flotation (DAF). the Board for the Doctorates Delft University of Technology.
    Vlaski, A., A. N. Van Breemen and G. J. Alaerts (1996). "Optimisation of coagulation conditions for the removal of cyanobacteria by dissolved air flotation or sedimentation." AQUA 45(5): 253-261.
    Wang, M., J. LI, S. Wei, Y. Ni and J. Liu (2011). Aerobic Granulation under Low Selection Pressure. International Conference on Environmental and Computer Science. Singapore IPCBEE. 19.
    Watson, S. (2004). "Aquatic taste and odor: a primary signal of drinking-water integrity." J Toxicol Environ Health A 67(20-22): 1779-1795.
    Westrick, J. A., D. C. Szlag, B. J. Southwell and J. Sinclair (2010). "A review of cyanobacteria and cyanotoxins removal/inactivation in drinking water treatment." Anal Bioanal Chem 397(5): 1705-1714.
    Wyatt, N. B., L. M. Gloe, P. V. Brady, J. C. Hewson, A. M. Grillet, M. G. Hankins and P. I. Pohl (2012). "Critical conditions for ferric chloride-induced flocculation of freshwater algae." Biotechnol Bioeng 109(2): 493-501.
    Yan, Y.-d. and G. J. Jameson (2004). "Application of the Jameson Cell technology for algae and phosphorus removal from maturation ponds." International Journal of Mineral Processing 73(1): 23-28.
    Yen, H. K., T. F. Lin and P. C. Liao (2011). "Simultaneous detection of nine cyanotoxins in drinking water using dual solid-phase extraction and liquid chromatography-mass spectrometry." Toxicon 58(2): 209-218.
    Yu, J., M. Yang, T. Lin, Z. Guo, Y. Zhang, J. Gu and S. Zhang (2007). "Effects of surface characteristics of activated carbon on the adsorption of 2-methylisobornel (MIB) and geosmin from natural water." Separation and Purification Technology 56(3): 363-370.
    Zabel, T. (1985). "The advantages of dissolved air flotation for water treatment." American Water Works Association 77(5): 42-46.

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