流行病學研究指出，慢性暴露於食物烹調中的油煙被認定是造成台灣女性肺癌的重要環境危險因子。Trans-trans-2,4-decadienal (ttDDE) 是從加熱的花生油油煙中萃取出的含量最多的物質 (51.6%)。本研究目的是以人類支氣管上皮細胞株 (BEAS-2B) 暴露此化學物質，並以二維膠電泳 (two-dimensional gel electrophoresis, 2D-GE) 顯示受到暴露後細胞內蛋白質量的變化情形。膠片影像用軟體分析後量有變化的蛋白質再利用質譜分析得到這些蛋白質身分鑑定。細胞受到 ttDDE暴露後有35種蛋白質的量是增加的，另12種的表現量是減少的。其中有35種蛋白質的身份已成功的鑑定出。這些受到ttDDE影響的蛋白質對照其功能的研究發現glutathione reductase (GR)、glutathione S-transferase (GST)、thioredoxin-like protein、 thioredoxin (TRX)、DJ-1 protein、cofilin、ribonuclease inhibitor and serum albumin precursor可能跟ttDDE 引發的氧化性壓力 (oxidative stress) 有關。此外，GST、 NAD-dependent alcohol dehydrogenase (ADH) 被認為可能跟ttDDE在生物體內的解毒機制有關。另外重要的是，retinoblastoma binding protein 7 (RBBP7) 在ttDDE暴露的細胞中可以看到量增加的變化，在肺癌病人的肺水中的蛋白質體中也觀察的到。因此，RBBP7與ttDDE毒性機制、肺癌的關係值得在未來加以研究證明。

　　Trans-trans-2,4-decadienal (ttDDE) is a widespread ,-unsaturated aldehyde found in food, water, and environmental pollutants. It is also the major part (51.6%) in neutral fraction of methanolic extract from fumes of heating peanut oil and is shown to have strongest mutagenicity. Data collected from an epidemiological case-control study has been suspected that exposure to fumes emitted from cooking oils when not reduced by an extractor appeared to be an important risk factor for lung cancer in Taiwanese woman nonsmokers. The objective of this work is to use proteomic approaches to study how this toxic chemical compound may alter the proteome in BEAS-2B normal human bronchial epithelium cells. The differences in the levels of proteins extracted from BEAS-2B cells with and without exposure to ttDDE, were investigated using two-dimensional gel electrophoresis (2D-GE) display. More than 600 protein spots were visualized after silver staining. The comparison between 2D-GE maps with ttDDE treated or controlled showed alterations of 47 proteins, of which 35 were up-regulated and 12 were down-regulated. The identities of the proteins with altered levels were determined by liquid chromatography-mass spectrometry analysis of in-gel tryptic digests and protein sequence database search. Thirty-five proteins were identified. These proteins were further explored using the bioinformatics tools from the Expert Protein Analysis System (ExPASy) for their cellular locations and functions. Among these proteins, glutathione S-transferase (GST), NAD-dependent alcohol dehydrogenase (ADH), may play roles in ttDDE detoxification pathways. Glutathione reductase (GR), GST, thioredoxin-like protein, thioredoxin (TRX), DJ-1 protein, cofilin, ribonuclease inhibitor and serum albumin precursor may be involved in oxidative stress induced by ttDDE treatment. Besides, Retinoblastoma binding protein 7 (RBBP7) is of special interest since it is also found in pleural effusion from lung cancer patients.

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