C型反應蛋白(CRP)為急性發炎時期表現蛋白，主要分為pCRP (MW=115 kD)及mCRP (MW=23 kD)兩種，醫學研究顯示CRP為心血管疾病及動脈血管硬化之重要指標蛋白，其中mCRP於近年來研究發現，相較於pCRP為更具活性之心血管疾病及動脈血管硬化誘發因子，因此量測血清中之CRP有助於及早判定發生心血管疾病之危險群，一般而言，ELISA為量測血清中CRP之常用方式，在此，利用表面電漿共振生物感測器，透過一連串的化學固定化方式，直接固定辨識抗體於感測器上，同時利用奈米強化表面電漿共振生物感測器提升感測器之靈敏度，欲達直接偵測血清中之pCRP及mCRP，利用表面電漿共振生物感測方式偵測擁有不需標定，及時偵測之優點。

C-reactive protein (CRP) is one of the most characteristic acute-phase proteins. The serum presence of this hepatic ~115 kDa protein of five identical subunits (pCRP) accompanies several diseases, such as cardiovascular disease (CVD) and atherosclerosis. In recent years, the modified CRP (mCRP) which exhibits different biological activities in the body has been regarded as a more powerful inducer than pCRP. Therefore, investigating and measuring the contents of pCRP and mCRP is helpful to determine the risk of develop ing CVD and atherosclerosis in advance. Conventionally, these inflammatory proteins are detected by high-sensitivity ELISA in blood serum. Now we develop a new method, an SPR-based (surface plasmon resonance) immunosensor, based on monoclonal antibodies, C8, 8D8, and 9C9 that are immobilized on the sensor chip through different immobilization ways for real-time and label-free CRP identification and detection.

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