本研究將電性鑑別法應用於甲基安非他命競爭免疫反應之檢測，利用晶片表面修飾技術將抗原或蛋白質固定於晶片表面，並結合微阻抗晶片與結合金奈米粒子抗體標定技術，以助於定性偵測待測檢體之免疫反應並定量阻抗量測分析結果鑑別。實驗中利用電感、電容、電阻量測儀(LCR meter)偵測阻抗晶片之電性訊號，藉由電極晶片阻抗變化來判斷競爭免疫反應發生之情況，並建立競爭免疫分析偵測系統。本實驗成功利用表面化學修飾方法增強蛋白質與晶片之接合能力，並將免疫檢測之檢體使用量降低至30 μL及競爭免疫反應偵測時間減少至13分鐘。另外可在低頻率時(100 Hz)檢測到較高鑑別度之阻抗電性訊號，再搭配濃度稀釋100倍的MET-GC作免疫偵測可降低其靈敏度至1 ng/mL。本研究利用表面修飾方法、阻抗分析晶片與奈米粒子免疫鑑別技術，達到快速偵測、低檢體使用量、低成本的目的，提供了毒品檢測與免疫分析一個全新的方向與思維。

This study, a novel micro-electrode biochip for immunoassay was applied to detect competitive immuno-reaction of methamphetamine, a gold nanoparticles (AuNPs) as a label of antibody to enhance immunoassay impedance quantitative analysis detection. Compared with traditional enzyme-linked immunosorbent assay (ELISA), we adopted self-assembled monolayer to immobilize of antigen or antibody on the chip surface, and detected the immuno-reaction signal of AuNPs to quantitative and qualitative analysis. The LCR meter instrument was used to detect the electric single on immune-impedance chip. It detected the impedance changes because of competitive immuno-reaction that was established a competitive immunoassay detection system. In this study, we used surface chemical modification method to modify the surface of immune-impedance chip. Then using the contact angle measurement instrument and the fluorescent labeled antibody method verify the results of chip modification. Only used 30 μL of the samples that the immune detection can be work in the immune detection system. The total detection time of the immunoassay was about 13 minutes. It has the best distinction under 100 Hz in this detection system, according to the relationship between the impedance and methamphetamine concentration. It was diluted 100 times of the MET-GC for the immune detection, the minimum signal detection of electrode chip was 1 ng/mL of methamphetamine. In this study, combined impedance changing detection and immunogold labeling technique in competitive immunoassay was a novel notion. Impedance measurement was performed for quantitative and qualitative analysis in the aim of decreasing detection time and lowering assay cost. It provided a new immunoassay method for drug-detection and medical analysis science.

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