鄰苯二甲酸酯常作為聚氯乙烯（PVC）產品的塑化劑，例如鄰苯二甲酸二甲酯（DMP），鄰苯二甲酸二乙酯（DEP），鄰苯二甲酸二異丁酯（DiBP）和鄰苯二甲酸二-2-乙基己酯（DEHP）皆是經常被使用的塑化劑。隨著塑化劑的大量使用和生產，民眾們更容易暴露到鄰苯二甲酸酯。因此可以通過生物偵測特定的代謝物來進行暴露評估。頭髮因為可以反映長時間的化學暴露時間，所以已越來越常被作為尿液的替代基質。因此開發一個利用頭髮作為非侵入性樣品來評估人體對鄰苯二甲酸酯暴露的生物偵測方法是非常必須的。在分析之前，將頭髮樣品去污並均質化。此外，使用5％TFA / MeOH（0.5：9.5，v / v）和15％TFA / UPW（1.5：8.5，v / v）兩種溶液進行固-液萃取，之後將兩者的萃取物結合且無需進行葡萄醣醛酸水解，然後使用連線固相萃取（SPE）液相層析串聯質譜法（LC-MS / MS）進行定量。最佳化後的方法針對線性、基質效應、偵測極限/定量極限、精密度以及準確度進行驗證。所有的目標代謝物均得到符合標準的結果。將此驗證方法應用於來自15個健康志願者的真實頭髮樣本之生物偵測研究中，8個代謝物的檢測頻率為100％。在本研究中發現疏水性單酯代謝物更容易摻入到頭髮中。根據本實驗獲得的結果顯示，本開發方法可以檢測頭髮中的鄰苯二甲酸酯代謝物，因此將支持將頭髮作為人類鄰苯二甲酸酯暴露生物監測的替代基質。

Phthalates, such as dimethyl phthalate (DMP), diethyl phthalate (DEP), and di-2-ethylhexyl phthalate (DEHP), are used as plasticizers for polyvinyl chloride (PVC) products. With the extensive usage and production, the public are more likely exposed to phthalates. Exposure assessment can be achieved by biomonitoring specific metabolites. Hair has been increasingly used as a complementary matrix to urine since it could determine the period of chemical exposure after several months to years. Thus, the requirement for the development of biomonitoring schemes using hair as a non-invasive sample to assess human exposure to phthalates is necessary. In this study, a method for the simultaneous measurement of multiple phthalate metabolites in hair samples was developed and validated in this study. Prior to the analysis, the hair samples were decontaminated and homogenized. Further, solid-liquid extraction with of 5% TFA/MeOH (0.5: 9.5, v/v) and 15% TFA/UPW (1.5: 8.5, v/v) then combined the extracts, which not be deconjugated, followed by quantitation using on-line solid phase extraction (SPE) liquid chromatography-tandem mass spectrometry (LC-MS/MS) was performed. The optimized method was validated as linearity, limit of detection/limit of quantitation, matrix effects, precision, and accuracy. The optimized method was implemented in the pilot biomonitoring study with authentic hair samples from 15 healthy volunteers. Detection frequencies of 8 metabolites were 100%. More hydrophobic monoester metabolites were found to be preferentially incorporated in hair. The obtained results suggest that the developed method can detect phthalate metabolites in hair, which supports that hair could be used as an alternative matrix in human phthalate exposure biomonitoring.

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