近年來，學者們發現吸附於奈米材料上的分子，接受特定波長光源後，拉曼訊號強度大幅提高，為微弱的拉曼散射開創一個嶄新的研究領域，表面增強拉曼散射(Surface-Enhanced Raman Scattering, SERS)。儘管表面增強拉曼散射是一種具有高靈敏度的分析技術，但要如何設計SERS系統以最佳提升增強效果是長久以來的研究目標。再者，雖然拉曼散射光譜能提供生物分子的結構與環境訊息，對生物學分析擁有很大的前景，但生物物質具有的非凡特性，如果能設計出可充分發揮生物特性的奈米材料並能提高SERS效果，將會是前所未有的挑戰與突破。
為了將生物物質與奈米材料結合以提高SERS成效，需要具備足夠的生物知識以及徹底了解SERS的理論基礎，例如SERS的化學增強是與待測分子與金屬基板之間的電荷轉移有很大的關聯，可透過生物特性去改變其電荷轉移以達到提升化學增強效果。在本研究中，我們合成出SiO2@Au shell，並將具有電子傳遞能力的膜整合脂質體(Membrane-integrated liposomes, MILs)與奈米材料結合，再經由多種實驗設計，包括修飾不同薄膜SERS實驗、物理混合實驗、二氧化矽殼阻斷實驗，證實了此具有金屬還原路徑之MILs可以透過其獨特電子傳遞能力增強SERS效果，並且也成功找出可有效運用其能力的SERS增強系統，SiO2@Au shell@MBA@MIL，二氧化矽包覆金殼可提供近紅外光的SERS應用，並由於金殼與4-MBA具有化學鍵結，可有助於化學增強，使MIL得以充分發揮其提供電子能力以提高SERS增強。最後經由分子的最高佔據分子軌道(HOMO)與最低未佔據軌道分子軌道(LUMO)以及金殼的費米能階之間能量差，揭示該系統是如何透過電子轉移的方式以提高SERS增強效果，有望進一步為SERS應用提供新思路。

Surface-Enhanced Raman Scattering (SERS) is a highly sensitive analytical technique, and designing SERS systems to maximize the enhancement effect has long been a research goal. Furthermore, while Raman scattering spectroscopy can provide valuable structural and environmental information for biomolecules, and has great potential for biological analysis, there has been limited research on how to utilize biomaterials in conjunction with nanoparticles to improve SERS efficiency. In this study, we synthesized SiO2@Au core-shell nanostructures and encapsulated them within membrane-integrated liposomes (MILs). It is demonstrated that these MILs, with their unique electron transfer capabilities, can effectively enhance the SERS effect via various experimental designs. On the basis of the results, we also successfully developed an optimized SERS enhancement system, SiO2@Au shell@MBA@MIL, and subsequent theoretical calculations elucidated how this system can improve SERS chemical enhancement through electron transfer processes. These findings provide valuable new insights to advance future SERS applications.

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