非傷寒沙門氏菌 (Non-typhoid Salmonella, NTS) 不僅為人畜共通傳染病，也是重要的食媒性疾病。人類常由於飲食不潔或生熟食交叉汙染而感染沙門氏菌，造成腹痛、腹瀉等腸胃道症狀。隨著抗生藥物的使用，抗藥菌株的演化與散佈，多重抗藥性的菌株已造成人類公共衛生的一大隱憂。國內過去在動物方面抗藥性的研究相對不足，為了更清楚釐清動物與人抗藥性散播的關係，本研究利用次世代定序技術 (Next Generation Sequencing, NGS) 將動物來源多重抗藥性Salmonella enterica subsp. enterica serotype Typhimurium (S. Typhimurium) 菌株BL10進行全基因解序，發現BL10共攜帶有22種抗藥基因，而且這些抗藥基因全位於4個質體。此外也利用BL10為參考菌株，比較4株其他動物及人類來源的S. Typhimurium菌株之抗藥基因與攜帶基因之質體，發現5株菌株分享共同的抗藥基因與4個質體，顯示人畜來源S. Typhimurium菌株間之流病關聯性。若無法有效地管制抗生素的使用，越來越嚴重的抗藥性問題會對人類健康及經濟造成嚴重威脅，唯有從環境、動物及人類防疫一體的角度進行抗生素使用的管制，才能確保大眾的健康。

Non-typhoid Salmonella (NTS) is not only a zoonotic disease, but also an important food-borne disease, usually causing diarrhea, gastroenteritis. With the spread of drug resistance, multiple drug resistant strains have caused a major public health concerns. In order to clarify the relationship between animal and human resistance distribution, we used next generation sequencing (NGS) to carry out the whole genome sequence of a multiple drug resistant strain BL10, and determined that the genome of BL10 have more than 20 drug-resistant genes, all of which located on plasmids. In addition, we also use BL10 as reference strain to analyze similar genes from four other multi-drug resistant strains. We found that all five strains have the constant resistance genes and plasmids, showing that the drug resistance genes might have spread between humans and animals. Results here suggest that if we do not effectively control the use of antibiotics, drug resistance will become a great threat to human health and the economy. Only from the perspective of “One Health” (environmental, animal and human) to control antibiotics, will we able to ensure the health of public.

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