本研究以雙碳鏈陽離子型界面活性劑dihexadecyldimethylammonium bromide（DHDAB）分別與單鏈陰離子型界面活性劑sodium hexadecylsulfate（SHS）、sodium dodecylsulfate（SDS）所形成之兩種三碳鏈離子對雙親分子（ ion pair amphiphile, IPA ）DHDA-HS和DHDA-DS為主材料，添加雙十六烷基磷酸（dihexadecyl phosphate, DHDP）使液胞帶負電，並以強制型製程製備出陰陽離子液胞，分析其物理性質、相轉移行為、雙層膜流動性及維他命E醋酸酯之包覆效率。此外，探討包覆維他命E醋酸酯後液胞雙層膜流動性之變化，並作細胞活性及溶血測試，以評估應用於藥物載體的可行性。
實驗結果顯示，純成分DHDA-HS可形成液胞，而足夠的碳鏈間凡得瓦力及液胞電性使其有良好的穩定性，由於SHS對水的溶解度較DHDAB高，故較易溶解出雙層膜而使液胞帶正電。添加雙鏈陰離子界面活性劑DHDP後，所形成之液胞的粒徑稍微下降，電性由正電轉變為負電，依然具有良好穩定性。螢光偏極化法的分析結果顯示，10 mol%及20 mol% DHDP添加下液胞雙層膜之相轉移行為非常相似，相轉移溫度則稍微下降。此外，由紅外光譜法分析碳氫鏈的自由度，添加10 mol%及20 mol% DHDP後，碳氫鏈排列呈不規則，此現象可能和DHDP及HS頭基間斥力有關。
在藥物包覆實驗中，DHDA-HS/DHDP （莫耳比8/2）與DHDA-DS/DHDP （莫耳比8/2）液胞系統包覆維他命E醋酸酯後，粒徑隨維他命E醋酸酯濃度增加逐漸上升至持平，而由於維他命E醋酸酯本身不帶電，故電性並無太大變化。比較兩個陰陽離子液胞系統對維他命E醋酸酯的包覆效率，DHDA-HS系統略高於DHDA-DS系統，可能和其疏水空間較多有關。紅外光譜法的結果顯示，包覆維他命E醋酸酯後，碳氫鏈排列呈規則特性，表示雙層膜流動性降低。此外，兩個陰陽離子液胞系統亦可包覆親水性藥物熊果素，證明兩個三鏈離子對雙親分子系統可形成液胞結構。
為了評估液胞包覆藥物後的應用性也進行細胞活性測試， DHDA-DS/DHDP（莫耳比8/2）液胞系統在包覆維他命E醋酸酯後，人類非小細胞肺癌細胞株（H1299）與正常人類上皮細胞（BEAS-2B）的存活率皆明顯提升，在液胞濃度低於0.5 mM時，細胞存活率皆高於80%，可視為低毒性。溶血測試結果也顯示液胞包覆維他命E醋酸酯後，溶血率有下降的趨勢。

In this study, two triple-chained ion pair amphiphiles, dihexadecyldimethylammonium-hexadecylsulfate (DHDA-HS) and dihexadecyldimethylammonium-dodecylsulfate (DHDA-DS) were prepared from double-chain cationic surfactant, dihexadecyldimethylammonium bromide (DHDAB), with single-chain anionic surfactants, sodium hexadecylsulfate (SHS) and sodium dodecylsulfate (SDS). With the consideration of drug delivery applications, dihexadecyl phosphate (DHDP) was added into DHDA-HS and DHDA-DS respectively. First, DHDA-HS can be fabricated as positively charged catanionic vesicles with the size of 130nm. With addition of DHDP, the size of DHDA-HS vesicle turned from 130nm into 100nm with negative charge. In addition, DHDA-HS/DHDP vesicle had high physical stability. Membrane characteristic was analyzed by fluorescence polarization and infrared spectroscopy. Infrared spectroscopy analysis revealed that increasing content of DHDP causes the arrangement of hydrocarbon chain more disordered, and fluorescence polarization analysis indicated the phase transition temperature was decreased. On drug encapsulation, DHDA-HS/DHDP vesicle and DHDA-DS/DHDP vesicle both could encapsulate hydrophobic drug, vitamin E acetate efficiently. In addition, with the successful encapsulation of hydrophilic drug, arbutin, it was proved that the DHDA-HS/DHDP and DHDA-DS/DHDP vesicles indeed possessed vesicular structures. BEAS-2B cells and H1299 cells were used to conduct the cytotoxicity assay for the vitamin E acetate encapsulated vesicles. The results indicated that the increased content of vitamin E acetate reduced the cytotoxicity and the hemolysis test also showed the vitamin E acetate encapsulation could cause hemolysis ratio decrease.

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