隨著人口增長和工業發展，大量燃燒化石燃料使二氧化碳(CO2)濃度在大氣層中快速增加。因此，發展CO2捕捉與封存技術是當務之急。光合作用已經被視為永續發展的CO2封存方法。光的利用為光合微生物如微藻與藍綠菌的生長和固定CO2一個重要因素，其影響光合生物反應器(PBR)的設計和操作。內部光照光合反應器(Internally radiated PBR)可以維持每個操作單元具有最佳化之光利用，因此在微藻與藍綠菌實場操作上具有發展前景。
在本研究中，將設計一組設計內部光照PBR並探討節螺藻(Arthrospira platensis)在其不同光條件下的生長特性進行。內部光照PBR的光照條件為內部以LED亮暗閃爍照明與外部以日光燈作為節螺藻生長之光源，其中外部日光燈光照條件為高光強度460 μmol m-2 s-1 與低光強度 360 μmol m-2 s-1;五個LED亮暗比(k)為1(LED連續光照)、0 (無LED光照)、0.9、0.8和0.7在1 Hz下閃爍。節螺藻之生長結果為:在外部高光強度下，單支LED k值為1、0.9、0.8、0.7和0的比生長速率(μ, d-1)分別為0.83、0.44、0.65、0.45和0.35;而在外部低光強度下，單支LED k值為1、0.9、0.8、0.7和0的μ (d-1)分別為0.26、0.24、0.18、0.21和0.23。兩隻LED k值為1和0.8下的μ (d-1)分別為0.79和0.59。根據閃爍理論推論表示，節旋藻之μ在LED k值閃爍下無促進生長之效應，因比生長速率顯示無光集成(no light integration)。然而節螺藻之生質體產率在外部高光強度下，單支LED k值為1、0.8和0.7之光照條件分別為0.24、0.21與0.22 g L-1 d-1，無明顯差別，顯示出閃爍下可獲得相似之節螺藻生質體產率。藻藍素(C-phycocyanin)和碳含量在上述所有光條件下無明顯變化，分別平均占藻乾重9.6%與54%。因此節螺藻之藻藍素產率和固碳速率由其生質體產率來決定高低。藻藍素產率於LED k值為1、0.8和0.7閃爍下分別為21、20和22 mg L-1 d-1。固碳速率於LED k值為1、0.8和0.7閃爍下分別為0.48、0.45和0.45 g L-1 d-1。最高固碳速率與藻藍素產率分別為0.48 g L-1 d-1和22 mg L-1 d-1。亮暗閃爍的效應可表現出節螺藻在藻藍素的產出與二氧化碳之固定具有節省成本方面之優勢，可在不增加LED的能耗而獲得相似的結果。

The concentration of carbon dioxide (CO2) has increased dramatically in earth’s atmosphere due to fossil fuels burning associated with increased population and industrialization. Therefore, the development of effective CO2 capture and storage technology is urgent and essential. Photosynthesis in microalgae and cyanobacteria has long been recognized as a suitable mean to sequester CO2. Light availability is an important factor for photobioreactor (PBR) design and operation, resulting in growth and CO2 fixation of microorganisms. Internally radiated PBR may maintain optimized light regime in each operation unit and is promising for scale up by increasing numbers of units. The light regime as flashing light in the PBR may enhance microalgae and cyanobacteria growth for increasing light utilization efficiency. Thus, the internally radiated PBR operated in flashing light was established.
In this study, the growth characteristics of cyanobacteria Arthrospira platensis (A. platensis) under various light conditions were investigated. We constructed an internally radiated bubble column PBR for A. platensis with LED light-dark flashing internally and fluorescent lamps illuminating externally: light intensities of fluorescent lamps were 460 μmol m-2 s-1 for high light intensity conditions and 360 μmol m-2 s-1 for low light intensity conditions; five LED light/dark fractions (k) were set to 1 (continuous illumination), 0 (without LEDs), 0.9, 0.8, and 0.7 at 1 Hz flashing. The specific growth rates (μ, d-1) under LED k = 1, 0.9, 0.8, 0.7, and 0 are 0.83, 0.44, 0.65, 0.45, and 0.35, respectively, at high light intensity conditions; 0.26, 0.24, 0.18, 0.21, and 0.23, respectively, at low light intensity conditions. The μ (d-1) under high light intensity condition with two LEDs at k = 1 and 0.8 are 0.79 and 0.59, respectively. Based on flashing effect theory, the specific growth rates indicate that there is no growth enhancement under LED light/dark flashing, which means there is no light integration enhancement. However, the biomass productivities under LED k = 1, 0.8, and 0.7 at high light intensity conditions show no differences, which are 0.24, 0.21, and 0.22 g L-1 d-1, respectively. The similar biomass productivities are obtained by applying LED light/dark fractions. The contents of C-phycocyanin (CPC) and carbon in dry A. platensis biomass show no differences under all light conditions, which are in average 9.6% and 54%, respectively. Therefore, the output of CPC productivity and carbon fixation rate is dominated by the biomass productivity. The CPC productivity at LED k = 1, 0.8, and 0.7 are 21, 20, and 22 mg L-1 d-1, respectively. The carbon fixation rate at LED k = 1, 0.8, and 0.7 are 0.48, 0.45, and 0.45 g L-1 d-1, respectively. The highest CPC productivity and carbon fixation rate are 22 mg L-1 d-1 and 0.48 g L-1 d-1, respectively. The CPC production and carbon fixation rate of A. platensis are maintained by the light/dark flashing. The cost saving by the light/dark flashing may be advantageous.

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