本研究利用光電二極體作為感測元件，結合側流層析免疫反應試紙，以膠體金的呈色訊號作為檢測標的，開發一具有高線性度、高靈敏度及穩定度之免疫呈色判讀系統，使免疫判讀於定量分析上更加精確。系統包含硬體及軟體兩部分，硬體方面裝載德州儀器MSP430G2553微控制器、光電二極體、LED光源、LCD液晶顯示器、整合式電路板及光學暗房。程式部分使用C程式語言搭配Code Composer Studio (CCS)整合開發環境撰寫訊號數位化處理與周邊元件控制程式。研究中以符合膠體金吸收光譜之光電二極體元件，進行光電訊號轉換並將其量化計算，以對側流層析免疫反應試紙Control、Test線(C、T線)上的膠體金接合濃度進行定量分析，並反推檢體之抗原含量。本研究導入田口方法進行量測最佳化實驗設計，結果顯示量測靈敏度提高31.9%，品質損失降低為57.2%。在對人類血紅蛋白樣本的量測實驗中，系統能正確分析C、T線數值，並建立抗原濃度與訊號量測值之關係曲線，該曲線可作為量化分析之依據，其決定係數(Coefficient of determination)達0.9663，在濃度極限偵測下，可量測最低濃度50 ng/mL，成功突破閾值100 ng/mL限制，並在重複實驗下仍可保持良好的穩定度(變異係數<15%)。

This thesis presents a highly sensitive system for detecting human hemoglobin (hHb) in feces. Combined with the lateral flow immunochromatographic assay, the system uses photodiodes to quantify hHb concentration by the colorimetric method. The system is composed of hardware and software parts, and the layout was designed by SolidWorks and AutoCAD. The analyzing programs are compiled by C language and loaded into MSP430G2553 microcontroller. After verification of each parts of the system, we find that the system detected hHb antigen from 450 ng/mL to 50 ng/mL successfully. The coefficient of variation of the experiment results is less than 15%, indicating good stability. In the Taguchi method, we studied the influence of photodiode feedback impedance, light intensity and light-shielding structure parameters on sensing the test-strip coloration. The analysis shows that the structure parameters were important factors in the detection system.

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