本研究利用石英晶體微天平法應用於免疫分析方法對糖化血色素(Hemoglobin A1c, HbA1c)及血色素(Hemoglobin, Hb)進行頻率檢測，利用化學修飾方法將抗體更穩定塗佈於晶片電極內，以助於偵側待測檢體免疫反應之頻率變化量測並定量分析糖化血色素濃度。實驗中將微質量感測系統結合微質量感測晶片偵測頻率訊號，藉由頻率之變化來分析討論免疫反應接合的多寡，建立免疫分析平台之微質量檢測系統，分別對糖化血色素與血色素做最佳抗體濃度之測試，再經免疫反應時間測試，糖化血色素與血色素之免疫反應時間皆於7分鐘內完成鍵結。實驗結果中，糖化血色素檢測範圍為1~5000 ng/mL，極限濃度為5300 ng/mL，血色素檢測範圍為10~20000 ng/mL，極限濃度為22000 ng/mL，利用整合型微質量檢測平台對混和糖化血色素與血色素之檢體進行濃度-頻率之轉換，成功對糖化血色素佔總血色素之濃度比例為2~16%區間之檢體做出實驗比例與實際比例之比較圖。本研究達到快速偵測且省去前置作業的製程時間與成本以及使用之檢體量只需6 µL，對於日後用於糖尿病之偵測分析提供一個新穎的層面加以分析討論。

This study presents a method to detect the concentration of glycated hemoglobin (HbA1c) and hemoglobin (Hb) by frequency variation value measurement. This experiment is based on the immunological principle, so we first found out the best antibody concentration that is coated on the chips to catch the antigens we want. In this experiment, cystamine medicines for electrode reaction zone can be chemically modified by the immune reaction time test, glycated hemoglobin and hemoglobin of reaction time are bonded to complete within seven minutes. Experimental results, glycated hemoglobin detection range of 1~5000 ng/mL and limit concentration is 5300 ng/mL, hemoglobin detection range of 10~20000 ng/mL and limit concentration is 22000 ng/mL. Finally, we successful detect the mix sample of glycated hemoglobin and hemoglobin with 2~16% (ratio of glycated hemoglobin in total hemoglobin).

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