將乾燥的台灣黃柏（Phellodendron amurense var. wilsonii）根部，以甲醇熱迴流萃取、過濾，濾液經減壓濃縮後，再利用氯仿與水進行分配萃取，得到氯仿層及水層。各畫分層利用管柱層析、薄層層析及再結晶等方法分離純化。目前共分離得到44個化合物，其中35個化合物經光譜分析，確定其化學結構為：Alkaloids：berberine、oxyberberine、13-hydroxy-8-oxoberberine、berberrubine、chilenine、dictamnine、γ- fagarine、skimmianine、haplopine、robustine、isoplatydesmine、1-methyl-2-dodecyl- 4(1H)-quinolone、canthin-6-one、noroxyhydrastinine、thalifoline、N-methyl-6,7- dimethoxyisoquinolone、methyl anhydroberberillate、anhydroberberillic acid等18個化合物；Coumarins：scopoletin、fraxidin、7-geranyloxy-6-methoxy-coumarin、phellocoumarin- A等4個化合物；Benzenoids：p-hydroxybenzaldehyde、methylparaben、vanillin、methyl p-hydroxycinnamate、4-methoxy-trans-cinnamic acid、methyl ferulate、3-hydroxy-4- methoxycinnamic acid、methyl isoferulate、3-[p-(3-methyl-2-butenyloxy)phenyl]-1- propanol等9個化合物；Limonoids：limonin、obacunone等2個化合物；Steroid：β- sitosterol；其他：glyceryl 1-butcosanate。其中phellocoumarin A為首次報導之新化合物，而13-hydroxy-8-oxoberberine、methyl anhydroberberillate與anhydroberberillic acid則為首次從天然界分離得到之化合物。

　　Thirty five compounds, including eighteen alkaloids:berberine, oxyberberine, 13-hydroxy-8-oxo- berberine, berberrubine, chilenine, dictamnine, γ-fagarine, skimmianine, haplopine, robustine, isoplatydesmine, 1-methyl-2-dodecyl-4(1H)-quinolone, canthin-6-one, noroxyhydrastinine, thalifoline, N-methyl-6,7-dimethoxyisoquinolone, methyl anhydroberberillate, anhydroberberillic acid; four coumarins: scopoletin, fraxidin, 7-geranyloxy- 6-methoxycoumarin; nine benzenoids: p-hydroxybenzaldehyde, methyl paraben, vanillin, methyl p-hydroxycinnamate, 4-methoxy-trans-cinnamic acid, methyl ferulate, 3-hydroxy-4-methoxycinnamic acid, methyl isoferulate, 3-[p-(3-methyl-2- butenyloxy)-phenyl]-1-propanol; two limonoids: limonin, obacunone; one steroid: β- sitosterol; one other: glyceryl 1-butcosanate were isolated from the roots of Phellodendron amurense var. wilsonii. Their structures were determined by 1H-, 13C-, 2D-NMR and MS spectroscopic analyses. Among them, phellocoumarin A is new and three compounds: 13-hydroxy-8-oxoberberine, methyl anhydroberberillate, anhydroberberillic acid are isolated from natural sources for the first time.

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