探討過敏反應的產生機制和發現過敏原，是目前眾所積極努力的方向。目前臨床上是以皮膚測試與特異性IgE檢測為主，此固然可檢測出患者各自的過敏原，但本質性的會有其涵蓋過敏原不足，且無法即時偵測出某些藥物過敏反應，是此類檢測上的瓶頸。過敏反應所產生的不適症狀，終究是病患體內的肥大細胞乃至嗜鹼細胞被活化後，釋放如組織胺等關鍵性發炎物質至組織或血液中所導致，故藉由偵測過敏反應所釋放出之物質，是檢測過敏反應發生與否之最直接的方法。基於此，本研究著力於一種可便於檢測組織胺的毛細管電泳電化學(CEEC)晶片的研發。我們利用微製程的技術，製作出具有電化學微小電極之玻片與十字形PDMS微管道(5 cm)，並共同經氧電漿處理及矽烷化反應修飾後接合而成。由偵測結果顯示，此修飾完成的微管道呈現4.5 x 10-4 cm2/Vs以上的電滲流力(EOF)，且CEEC晶片在7天內均可維持在極佳的穩定度。在組織胺之電化學活性探究上，先以循環伏安法量測，由結果得知組織胺於金工作電極上，約在+0.8 V (vs. Ag/AgCl)附近，可偵測到其氧化之電流值且與濃度呈正相關。最後在晶片系統測試時，設定予100 V/cm的電場進行30 sec注入，繼之以同樣電場來進行毛細管電泳分離，此時在170 sec即可偵測到組織胺的氧化電流訊號。此系統對組織胺的檢測極限可達10-4 M，其線性範圍在0.5 ~ 2 mM之間 (R2=0.99)。本團隊之CEEC晶片系統原已約可在130與160 sec分別偵測出Dopamine與Catechol的訊號，加上本研究之展進，將有利於較複雜的人體血清乃至於細胞級樣本之偵測。

　Recently, numerous researchers investigated the mechanism of the allergic reaction aggressively in order to identify possible allergens and search for the effective treatments. The clinical diagnosis of allergy is based on skin test or the determination of immunoglomulin E (IgE) level. However, it is difficult to immediately identify the allergens due to limited allergens which were available in the lab tests used clinically now. The cross-linking of IgE between mast cells and basophils is known to cause the release of many mediators which could initiate the acute allergic reaction. For this reason, our research has focused on the development of capillary electrophoresis chip with electrochemical detector (CEEC) for detecting allergic mediators such as histamine. On the basis of the concept and technology of microfabrication, a micro-electrochemical chip and PDMS micro-channel were fabricated by MEMS process. The CEEC chips were modified by O2 plasma treatment and silianization for altering the hydrophobicity of PDMS surface, enhancing electroosmotic flow and stabilizing the flow over time. The stability of the resulting CEEC chip we studied over a span of 7 days as was higher than 4.5 x 10-4 cm2/Vs. In the study of electro-oxidation behavior of histamine, it was oxidized at gold electrode at +0.8 V. When the EOF of the chip is around 4 x 10-4 cm2/Vs, histamine was detected at 170 sec after separation. Histamine responded linearly from 0.5 to 2 mM and the limit of detection is 10-4M. It was concluded that, a stable detecting system has been developed. According to our previously research, dopamine and catechol were detected separately around 130 sec and 160 sec after separation, adding up our present study, many advantageous features will make it attractive to clinical investigators as a powerful adjunct for the diagnosis and treatment of allergy in the future.

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