本實驗室發現之蝦類高變異性分子 Dscam 的研究顯示，蝦體免疫具有專一性識別不同病原體的特性。在蝦體中，Dscam 存在兩種形式:具有胞質尾的鑲膜型 Dscam 及不具胞質尾的胞泌型 Dscam；胞外區域可經由選擇性剪切產生多變性以參與病原體辨認，而在胞質尾部分之多變性推測可驅使不同下游訊息反應。由於蝦類 Dscam 對於病原體所誘發的免疫特性及反應過程並未被討論，因此本研究乃以白點症病毒作為刺激物，觀測 Dscam 在感染過程的表現模式。結果顯示，在免疫相關組織所表現的鑲膜型 Dscam，其胞質尾帶有免疫相關功能性區域的比例遠超過其他組織，顯示其參與蝦體免疫功能的可能性。在全蝦體組織之整體 Dscam 的變化情形則顯示，病毒感染可誘發蝦體血細胞產生胞泌型 Dscam，推測胞泌型 Dscam 可被分泌進入血淋巴中以識別病原體。本研究接續以螯蝦作為實驗動物以進行長期觀察，從中發現在感染病毒後的存活者，其血漿中可偵測到較高量的 Dscam。利用膠體過濾分析，顯示其可能具有形成多聚體特性。根據以上結果，本研究推測，蝦類 Dscam 在剪切機制、免疫反應及分子結構上，相似於脊椎動物抗體 IgM，也具有相似的作用機制，並參與在白蝦對抗白點症病毒過程中。

Until now, the mechanism of shrimp immune response still not fully understood. Previously, we found that shrimp has a hypervariable molecule called Down syndrome cell adhesion molecule (Dscam) which can recognize different pathogens. Based on Dscam structure and immune properties, Dscam has been proposed to be an antibody-like molecule in arthropod. In this study we use white spot syndrome virus (WSSV) as pathogen stimulator to observe the Dscam’s cytoplasmic tail variants expression in various tissue after WSSV infection. The results show that cytoplasmic tail element 1A which consist of an endocytosis motif was expressed mainly in immune-related tissues, while element 1B which lack of this motif and expressed mainly in non-immune related tissues.So, cytoplasmic tail element 1A may be involved in Dscam immune function. Furthermore, the membrane-bound and tail-less Dscam expressions were analyzed in various tissue after WSSV infection. In hemocyte, tail-less Dscam can be induced after 36 hour post WSSV infection. Thus, we hypothesized that high amount of tail-less Dscam can be secreted into hemolymph after pathogen challenge. In order to test the hypothesis, hemolymph from two months crayfish survivors after WSSV infection were collected. High level Dscam expression was detected in the WSSV survivor’s hemolymph. To investigate whether Dscam has similar property with IgM. We use gel filtration to analyze the possibility of Dscam forms polymer in hemolymph. The result shows that Dscam may form dimer or heterpolymer in the hemolymph. Taken together, based on Dscam cytoplasmic tail splicing mechanism, the secretion ability of Dscam after pathogen challenge and the polymer structure, this study suggested that Dscam may be an IgM-like moleculer in arthropod..

莊雅竹，白蝦類專一性免疫作用分子 Dscam 之蛋白質特性分析，國立成功大學生物科技研究所碩士論文，2011。

洪欣宜，由細菌性及病毒性病原體所誘發隻白蝦 Dscam 探討其可能免疫作用機制，國立成功大學生物科技研究所碩士論文，2012。

黃芷菡，病毒誘發之高變異 Dscam 以類調理素方式參與螯蝦免疫記憶性，國立成功大學生物科技研究所碩士論文，2013。

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