近年來由於河川水源及集水區受污染的情況日益嚴重，而易引起水庫的優養化(Eutrophication)。優養化的水質，很容易造成藻類的蔓延與滋長，藻類之大量生長會影響水質的色澤和臭味。藻類大量繁殖的水源，易造成水處理的困難，即使經過傳統的淨水處理程序後，有時仍然帶有一些顏色，給人不愉快的視覺。此外，某些藻類本身也具有毒性，可能危害人體健康。

本研究目的主要是以氧化劑進行藻體氧化，觀察並探討藻體氧化前後之差異，以純藻：綠藻(Chodatella sp.)和矽藻(Nitzschia sp.)分別配製人工懸浮藻液。對其分別以高錳酸鉀及臭氧氧化後，針對氧化前後藻體胞外物變化之原因，進行下列試驗以進一步尋找可能之因素：分別以光譜分析與螢光強度測定，進行藻體胞外物之定性、定量；測定界達電位以觀查藻體表面電位之改變；以高效液相層析儀進行藻體胞外物(Bound EPS)分子量分析；觀察藻體氧化前後凝聚現象之變化。

研究結果顯示，綠藻(Chodatells sp.)和矽藻(Nitzschia sp.)以不同高錳酸鉀添加劑量進行氧化時，可以發現綠藻與矽藻之螢光強度皆有隨劑量增加而提升螢光強度的趨勢，高錳酸鉀能刺激藻體細胞分泌胞外物質。以臭氧低劑量進行藻體氧化時，藻體螢光有明顯增加的趨勢，然而臭氧劑量持續增加時，螢光強度有減少的現象。所以認為臭氧在低劑量下能刺激藻體，使胞外物質增加，當臭氧劑量較高時，胞外物可能被臭氧降解而減少。

在紅外光譜分析方面，綠藻與矽藻皆發現有胜鍵的存在，顯示蛋白質為藻體胞外物質重要成分之一。以矽藻而言，則可以發現矽類官能基(Si-O-Si、Si-O-C)為主要成分之一。
經實驗分析綠藻的分子量約為6000；矽藻的分子量約為9700，使用高錳酸鉀或臭氧進行氧化試驗後，其胞外物分子量並沒有顯著的變化。

以高錳酸鉀對綠藻進行氧化，其藻體間凝聚現象有隨添加劑量增加而提高的情形；以臭氧進行氧化試驗，在較高劑量2 mg/L時，方才觀察到藻體間稍有凝聚現象。在矽藻方面，經過高錳酸鉀和臭氧氧化後，矽藻凝聚現象的變化皆不甚明顯。

Eutrophication is becoming a more serious problem in Taiwan due to the discharge of pollutants into rivers and reservoirs. The growth of algae may affect water quality, such as causing color and odor problems. Some species of algae may even release toxin. This kind of water is difficult to treat by conventional treatment process. Unpleasant color may still be present in the treated water.

The main purpose of this research is to study the changes in extracellular polymeric substances of algae after treatment by two oxidants, namely potassium permagnate (KMnO4) and ozone. Synthetic algal suspensions with pure culture of Chodatella sp. or Nizschia sp. was used. Several tests were done to observe those changes: Fourier transformed infrared spectroscopy (FTIR) and florescence intensity was used as qualitative and quantitative analysis of EPS, respectively. Zeta potential of the algal surface was also measured. The molecular weight (MW) of the bound EPS was determined with high performance size exclusion chromatography (HPSEC). Also, the change in aggregation of algae was observed by microscope.

Results showed that there was a positive correlation between dosage of KMnO4 and fluorescence intensity for Chodatells sp. and Nitzschia sp.. This shows that KMnO4 may stimulate the production of EPS. Similar trend was found when using low concentration of ozone as oxidant. But when ozone dosage became higher, the fluorescence intensity decreased.It is speculated that EPS may be degraded by higher concentration of ozone.

In FTIR analysis, peptide bonds were found in both Chodatell sp. and Nitzschia sp.. This shows that protein may be one of the most important components of EPS. Silicon containing functional groups (Si-O-Si, Si-O-C) were also found in Nitzschia sp..
Molecular weight of Chodatells sp. is about 6000, and about 9700 for Nitzschia sp.. After treatment with potassium permagnate and ozone, no significant change was found in the MW of their EPS.

The Chodatells sp. became more aggregate when the dosage of KMnO4 increased. When treating with ozone, aggregation was only observed when ozone concentration was higher than 2 mg/L. For Nitzschia sp., the aggregation phenomenon was not obvious after treating with KMnO4 and ozone.

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