下肢缺血是一種主要由周邊動脈疾病(PAD，Peripheral arterial disease)引起之症狀，其成因為血管堵塞造成血流降低，以至於無法提供身體組織養分以及代謝廢物，最終使該區域組織壞死、喪失功能。在血管堵塞好發部位中，頸動脈堵塞會造成腦部之血流阻斷，會引起腦中風；冠狀動脈堵塞造成心肌梗塞；而在下肢處產生血管堵塞，則引起下肢缺血和造成組織壞死。過去實驗室研究中，在小鼠下肢缺血治療研究上有顯著之成果。對於下肢缺血研究多為小鼠及兔子，使用大動物部分相對較少。而在實驗動物當中，豬的血管結構及生理和人類相似度為最高的，因此選用豬進行下肢缺血治療研究，相較其他動物有其優勢。然而目前豬下肢缺血模式中，進行缺血手術後難以產生類似臨床上下肢缺血病患之症狀。於是，本研究目的為建立有症狀的豬下肢缺血模式。在開創之蘭嶼豬下肢缺血模式中，我們發現以糖尿病豬搭配外腸骨動脈至脛骨動脈栓塞手術，能使缺血下肢產生跛行現象。且手術後第四週血管攝影中顯示外腸骨動脈持續缺血，在都卜勒超音波結果顯示栓塞處動脈無血流訊號。前後肢血壓比中缺血下肢數值較控制組下肢低0.1，並維持四週。此建立的下肢缺血動物模式後續將能應用於疾病治療之研究。

Hind limb ischemia is a symptom mainly caused by Peripheral arterial disease, which causes blood flow to be reduced due to clogging of blood vessels so that it is unable to provide body tissue nutrients and metabolic waste, which eventually causes tissue necrosis and loss of function. In the vascular occlusion area, blockage of the carotid artery can cause blood flow in the brain to block, which can cause a stroke; coronary artery occlusion causes myocardial infarction; and vascular occlusion in the lower limb causes hind limb ischemia. In the past, our laboratory had achieved remarkable results in the ischemic limb treatment in mice. Most of the studies on hind limb ischemia were mice and rabbits, and the use of large animals was rarely. Among the experimental animals, the vascular structure and physiological of porcine were highly similar in the human. Therefore, the porcine application for hind limb ischemia treatment had advantages over other animals. However, the porcine hind limb ischemia model is difficult to produce symptoms like clinical after ischemic surgery. The purpose of this study was to establish an asymptomatic hind limb ischemia model in pigs. We found that diabetic pigs with external iliac artery to tibial artery embolization surgery can cause claudication in the ischemic limb. In the fourth week after the operation, the angiography showed continued ischemia of the external iliac artery, and the doppler ultrasound showed that there was no blood flow signal at the embolism site. The hindlimb-forelimb index value of the ischemic limb is lower than that of the control group and maintained for four weeks. This established hind limb ischemia model will be applied to the research of disease treatment in the future.

李易，DIB 於糖尿病下肢缺血小鼠治療之研究，國立成功大學生物科技研究所碩士論文，2016。

梁致文，蘭嶼豬糖尿病模式建立與傷口癒合研究，國立成功大學生物科技研究所碩士論文 ，2017。

鄭紫妃，改良之DIB膠於小鼠下肢缺血之治療，國立成功大學生物科技研究所碩士論文，2016。

簡崇美，糖尿病下肢缺血小鼠之治療，國立成功大學生物科技研究所碩士論文，2014。

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