硫氰酸(SCN-)是煉焦廢水中的主要污染物質之一，工業上常利用活性污泥法處理硫氰酸廢水。活性污泥槽常因環境發生變異，影響硫氰酸分解菌之分解能力，因而影響活性污泥槽的處理效率。利用分子生物方法探討硫氰酸分解菌群，追蹤硫氰酸分解菌之消長，可以即時監測活性污泥槽中硫氰酸之分解效能。
在本研究之汙泥樣本取自台灣南部某鋼鐵廠之VC755B活性污泥槽，利用16S rRNA gene分子選殖技術，建立活性污泥槽之菌群分子選殖資料庫，並利用末端螢光標定限制片段多型性分析法（T-RFLP）即時監測活性污泥槽中硫氰酸分解菌群的結構變化。
在活性污泥廢水的選殖資料庫中，得知污泥中硫氰酸的主要分解菌屬於Thiobacillus，在clone library中佔26.6%。由T-RFLP結果顯示，Comamonas (30.5%)為活性污泥中最顯著的菌種。活性污泥中主要的菌群包括Thiobacillus(5.3%)、Pseudomonas(19.2%)、Comamonas(30.5%) 和Bacteroidetes(15.8%)。將活性污泥經硫氰酸培養基培養後(九日內馴化三代)，微生物族群發生變化。Comamonas被偵測到有消失的情形，而活性污泥中的Thiobacillus由5.3%升高到39.2%，Pseudomonas菌群也由19.2%提升到23.9%。因此藉由T-RFLP的分析，可以有效的偵測到活性污泥樣本中微生物結構的變動。

Thiocyanate is one of the contaminants in coking waste water, and it usually treated by activated sludge system. When environment condition of activated sludge reactor is changed, the SCN- degrading ability will be influenced. Therefore, cultivation-independent methods were used to study the composition of thiocyanate degrading bacteria and monitor the changes of bacteria in the activated sludge reactor.
In this study, the sludge samples were taken from VC755B activated sludge reactor of a steel plant in south Taiwan. 16S rRNA gene was used to construct to the clone library of activated sludge and monitor the dynamic changes of bacterial community by terminal-restriction fragment length polymorphism (T-RFLP).
In 16S rRNA gene clone library of activated sludge, Thiobacillus (26.6%) was the dominant thiocyanate-degradation bacteria in the reactor. T-RFLP electropherograms revealed Comamonas (30.5%) was the dominant bacteria in activated sludge. The major bacteria in the activated sludge included Thiobacillus(5.3%), Pseudomonas(19.2%), Comamonas(30.5%) and Bacteroidetes(15.8%). The sludge was enriched with SCN- (3 subculture in 9 days) and the microbial community. The disappearance of Comamonas was detected and Thiobacillus increased obviously changed in the sludge was obvious from 5.3% to 39.2%. Pseudomonas increased from 19.2% to 23.9%. The changes of microbial community could be detected by T-RFLP.

趙幼梅和陳文昌，探討煤化學脫硫場尾氣成分對排放水SCN-之影響。中國鋼鐵公司研究報告。高雄市。2001
吳哲宏，處理煉焦廢水活性污泥系統中SCN-分解菌群基因鑑定。中鋼公司委託研究計畫。高雄市。2006
申忠哲，處理煉焦廢水之活性污泥槽中硫氰酸分解菌的族群結構之研 究 。國立成功大學生物學研究所碩士論文。2004
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