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研究生：	陳鴻瑩 Chen, Hung-Ying
論文名稱：	運用介電泳力原理並具多重/連續分類功能之微分類器研究 The Study of DEP-based Microsorter for Multiple/Continuous Particle Sorting
指導教授：	蕭飛賓 Hsiao, Fei-Bin 呂宗行 Leu, Tzong-Shyng
學位類別：	博士 Doctor
系所名稱：	工學院 - 航空太空工程學系 Department of Aeronautics & Astronautics
論文出版年：	2006
畢業學年度：	94
語文別：	英文
論文頁數：	131
中文關鍵詞：	介電泳力、微分類器
外文關鍵詞：	dielectrophoretic, microsorter
相關次數：	點閱：86 下載：1
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　　本研究之目的在於探討應用介電泳力之原理研發微分類器。研究動機，有鑑於在生物科技領域及醫藥研究中所必須的重要步驟之一，是能將所要研究分析的細胞或粒子，從一群混和物中分離分類出來。本研究首先搜尋學術界在此方面之探討而發現介電泳力廣為接受為細胞分離之重要機制而且在分類過程中不會損害供研究之樣品活細胞。
　　根據介電泳力的理論，本研究提出一創新之微分類器設計。此設計運用兩組電極產生介電泳力電場，能有效地將連續注入微管道的乳膠粒子先做聚焦在分類器第一階段，並排列成一列，通過第二階段分類器產生偏移現象，對於相同介電特性但不同大小粒子，因受介電泳力而偏移到不同的橫向位置，以便收集在不同之收集槽，以此來作分類。整個設計與步驟均由數值模擬與實驗來驗証分類效果。
　　數值模擬與實驗結果作比較，印証了DEP之理論與模擬軟體之可信度。更重要的發現是証明了本研究所提出之微分類設計概念，是適切可行且獨具特色的。本研究之結論幾點創新之貢獻，並建議未來可進一步研究之方向。

　This study is an attempt to research on microsorters by incorporating Dielectrophoresis (DEP) theories. The research was inspired by the impressing need, in the field of biotechnology and medical applications, of the ability to sort out the cells of interest from the sample mixture. The study started with related literature review regarding DEP force and microsorting mechanism. It was confirmed that DEP, in biotechnology, serves as an appropriate mechanism in manipulating and sorting cells for no risk to cause damage to the sample.
　Based on the DEP theories, an innovated microsorting design was proposed in this study. The design employed DEP force to both focus and continuously sort the particles, through the contraction electrode as the first section and parallel electrode with different length as the second one. The designed model and procedures were carried out and verified through numerical simulation (CFD-ACE+) and experiment.
　The simulation and experimental results were reported and compared. The experiment results are agreement with the simulation results. Most importantly, the research finding indicated that the proposed design of microsorter was proved to be feasible, appropriate, and unique. The study concluded with the contribution to the field of microsorting and the implication for further research.

ABSTRACT…………………………………… i
CONTENTS……………………………………	iii
LIST OF TABLE……………………………………………………… vii
LIST OF FIGURE……………………………………………………	viii
NOMENCLATURE……………………………………………………… xvi
I　INTRODUCTION……………………………………………… 1
1 Research on DEP as Microsorting Mechanisms……………………	2
2. Research on the Application DEP………………………………… 3
3. Rational and Scope of the study………………………………………… 5
II　THEORETICAL BACKGROUND OF DEP FORCE………………… 7
1 DEP Force……………………………………………………………… 7
2 Stokes’ Drag Force vs. DEP Force………………………………………	10
3 Lateral Force vs. DEP Force…………………………………………… 11
4 The Simulation Software Based on DEP Theories……………………… 14
III　SIMULATION TOOL……………………………………………………… 15
1 Flow Module …………………………………………………………… 15
1.1 Mass Conservation………………………………………………… 16
1.2 Momentum Conservation………………………………………… 16
2 Spray (Particle) Module………………………………………………… 17
3 Drag Coefficient………………………………………………………… 18
4 Electric  Module………………………………………………………… 19
5 Dielectrophoretic Force Module………………………………………… 20
IV　Chip FABRICATION AND EXPERIMENT SET-UP…………… 24
1 Chip Fabrication………………………………………………………… 24
1.1 Fabrication of the Electrode Parts………………………………… 24
1.2 The Channel Formation and Chip Bonding……………………… 25
1.3 Chip Packaging and Tubing……………………………………… 26
2 Experiment Sep-up……………………………………………………… 26
2.1 Optical System…………………………………………………… 26
2.2 Electrical Excitation System………………………………………	27
2.3 Fluidics System…………………………………………………… 27
2.4 Sample Preparation……………………………………………… 28
V　CHIP DESIGN AND THE COMPARISM SIMULATION AND EXPERIMENT…29
1 The Micron Flow-sorter of the 1st Generation—with Uneven Parallel Electrode Design………………………………………………………………	29
1.1 The Construction of the Microsorter Design……………………… 29
1.2 Simulation of the Microsorter Design……………………………… 31		
1.3 Summary of the 1st generation of DEP micron flow-sorter with Uneven Parallel Electrode……………………………………… 37
2 The Micron Flow-sorter of the 2nd Generation—Using Convergent Electrodes for Particle Holding, Separating, and Focusing…………… 38
2.1 The Construction of the Microsorter Design…………………… 38
2.2 Simulation of the Microsorter Design…………………………… 39		
2.3 Summary of the 2nd Generation Microsorter Using Convergent 
Electrodes for Particle Holding, Separating, and Focusing………… 44	
3 The Micron Flow-sorter of the 3rd Generation-- the multiple DEP microsorter… 46
3.1 The Construction of the Microsorter Design………………………… 46
3.2A Simulation Study of the Microsorter — the Contraction Electrode Section… 50
3.3A Experiment Results of the Contraction Electrode Performance…… 59
3.2B Simulation Studies of the Microsorter — the Uneven Parallel Electrode…… 61
3.3B. Experimental Results of Particle Separation……………………… 66
4 The 4th Generation of DEP Microsorter with Trapezoidal Deflector…… 68
4.1 The 4th Generation of DEP Microsorter with Trapezoidal Deflector…… 68
4.2 Simulation of  distribution of the Trapezoidal deflector………69
4.3 Experiment Result of the Trapezoidal Deflector…………………… 73
VI　CONCLUSION…………………………………………………………… 75
1 Conclusion of the Study……………………………………………………	75
2 Recommendation for Further Research…………………………………… 76
REFERENCE………………………………………………………………… 78
TABLES……………………………………………………………………… 87
FIGURE……………………………………………………………………… 91
                                    

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