本研究主要是探討綠色螢光蛋白濃度與螢光強度之間的關係，以便應用於使用巨大原生質體之生物取像系統。首先將gfp基因植入在pET21a質體，以BL21為宿主大量表現綠色螢光蛋白。
純化定量出綠色螢光蛋白後，分析純化後綠色螢光蛋白在螢光光度計之螢光強度，與巨大原生質體內綠色螢光蛋白在螢光顯微鏡下和螢光光度計下的螢光強度表現結合，確立巨大原生質體以螢光顯微鏡取得螢光強度與以螢光光度計取得之螢光強度之關係，可以使巨大原生質體在螢光顯微鏡下觀察分析後，直接藉由此關係來推估所生成的綠色螢光蛋白濃度，不僅跳脫以往只是定性的觀察培養中螢光變化外，更可以定量出螢光蛋白濃度的高低，利用此結果，可以提升巨大原生質體之生物取像系統的發展潛力，更可以精確地標的定量出目標蛋白的含量。

The correlation between green fluorescence protein concentration and intensity is studied in this research. We can use this correlation in the bioimaging system of giant protoplast. First we inserted green fluorescence protein gene (gfp) into pET21a, and we use Escherichia coli BL21 to express green fluorescence protein massively.
Firstly, Fluorescence Spectrophotometer was used to quantify and analyze the fluorescence intensity of purified green fluorescence protein. Secondary, the fluorescence intensities of green fluorescence protein in giant protoplasts were determined by Fluorescence Microscopy and Flourescence Spectrophotometer, respectively. Using this correlation, the concentration of green fluorescence protein produced by the giant protoplasts could be quantified when we directly analyze the photos from Fluorecence Microscopy. A correlation between the values of green fluorescence protein fluorescence intensity in giant protoplasts which was determined by Fluorescence Microscopy and was determined by Flourescence Spectrophotometer was built-up by comparing with the value of purified green fluorescence protein fluorescence intensity.
We established a giant protoplast-bioimaging system in which target protein not only can be qualitative but also can be quantitative.

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