微小核仁蛋白(MSP58/MCRS1)核蛋白，參與在許多細胞功能中並扮演重要角色例如：轉錄的調控、細胞增生、細胞轉型與細胞衰老等。在我們實驗室過去曾利用酵母菌雙雜合分析，將MSP58當成誘餌發現了許多重要的蛋白質與MSP58有著交互作用.。並且在實驗室過去的研究成果發現到，MSP58能與SWI/SNF染色質重組複合體的成員 BRG1交互作用並共同調控p53/p21所主導的細胞衰老。除了BRG1，我們更近一步的發現另一個有可能與MSP58交互作用的重要蛋白質BRD7，BRD7是一個蛋白質序列中含有bromodomain區域的核蛋白，並隸屬於SWI/SNF-PBAF染色質重組複合體中。過去對於BRD7的研究指出，其扮演著腫瘤抑制者的角色並能結合上染色體中乙醯化的組織蛋白同時調控基因的轉錄，而BRD7能透過調控許多細胞存活機制抑制細胞的增生例如:ras/MEK/ERK、Rb/E2F與Wnt訊息傳遞路徑等，並且BRD7在許多的癌症中呈現低表現的情況如:鼻咽癌、前列腺癌、乳癌、卵巢癌等。有趣的是，BRD7也曾被報導能調控p53的基因轉錄活性並且與p53交互作用，調控p53所主導的致癌基因誘導的細胞衰老(OIS)。在我的實驗中以利用生化的方式確認了MSP58與BRD7的交互作用，而在未來我們將繼續探討MSP58與BRD7所組成的複合體參與在細胞增生之訊息傳導路徑中的角色與其分子機制，本次的實驗結果將提供MSP58與BRD7複合體參與在腫瘤增生的資訊。

58-kDa microspherule protein (MSP58) is a nucleolar protein and plays an important role in a variety of cellular function including transcriptional regulation, cell proliferation , transformation and senescence. We conducted yeast two-hybrid screening using MSP58 as bait and identified several interacting partners. In a previous study, we demonstrated that MSP58 associates with BRG1 and induces cellular senescence through the p53/p21 pathway. In addition to BRG1, BRD7 (Bromodomain Containing 7), is another candidate protein. BRD7 is a bromodomain containing protein, which is a subunit of PBAF-specific SWI/SNF chromatin remodeling complexes. It has been shown that BRD7 acts as a tumor suppressor by binding the acetylated histones in chromosomes. The expression of BRD7 was down-regulated in nasopharyngeal carcinoma, prostate cancer, breast cancer, and ovarian carcinoma and it could inhibit cell growth through multiple mechanisms. For example, BRD7 suppressed the ras/MEK/ERK, Rb/E2F and Wnt signaling pathways. Interestingly, BRD7 have been reported to interact with p53 and is required for the efficient induction of p53-dependent oncogene-induced senescence. We have confirmed the interaction between MSP58 and BRD7 biochemically. We will further explore the roles and molecular mechanisms of MSP58/BRD7 complex in cell proliferation signaling pathway. Results of this study may provide information for the roles of MSP58 and BRD7 in tumorigenesis.

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