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研究生: 劉孟麟
Lau, Mang-Lun
論文名稱: 香環燃煙中PAHs成份特徵與急毒性研究
Characteristics of PAH Emissions and Acute Toxicity of Incense Coil Smoke
指導教授: 林達昌
Lin, Ta-Chang
學位類別: 碩士
Master
系所名稱: 工學院 - 環境工程學系
Department of Environmental Engineering
論文出版年: 2005
畢業學年度: 93
語文別: 中文
論文頁數: 143
中文關鍵詞: 急毒性香環重金屬
外文關鍵詞: microtox, SVOCs, PAHs, metals, incense coils, acute toxicity
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  •   本研究挑選市面上最普遍的五種香環,對其中的成份作元素及重金屬分析,且採用經PS-1採樣器改裝的採器設備,於燃燒香環時以XAD-2樹脂和石英濾紙將氣相與固相半揮發性有機物(SVOCs)吸附並且萃取出,以GC/MS對16種PAHs作化學分析,並以Microtox對淨化前之SVOCs及淨化後之萃取液進行急毒性測試。
      本實驗結果發現各種香環之16種PAHs排放係數分佈皆很相似,氣相以二環及三環PAHs為主,固相則以三至七環PAHs為較多,但各種香環之PAHs產生量則各有差異,其中氣相PAHs以老山香環含量為最高,料香環則為最低;固相方面則以中藥香環含量為最高,料香環亦為最低。
      元素分析結果發現懸浮微粒量及香灰量兩者皆與碳含量有相關;各香環重金屬分析結果皆以鈣含量為最高,其次為K、Al、Pb、Fe、Na;在Microtox急毒性測試中發現香環產生燃煙之氣相SVOCs方面以中藥香環毒性為最高,老山香環毒性最低;燃煙之固相SVOCs中以沉香環之為毒性最高,中藥香環則是毒性最低;若以SVOCs氣固相之總和毒性結果顯示,毒性之順序排列為沉香環>新山香環>中藥香環>料香環>老山香環。此外,氣相之毒性與香環中鈉元素含量呈負相關。在PAHs之氣相部分發現新山香環之毒性為其中最高,而老山香環亦為毒性最低,固相部分以中藥香環毒性最高,老山香環則是最低;而總SVOCs之毒性比淨化後之萃取物毒性高出1~6倍,表示在總SVOCs中可能含有急毒性更高之物質存在,但它們在淨化過程中被除去了,因此建議同時針對在淨化過程中被除去的SVOCs部分作化學分析與毒性測試,以更了解香環燃煙中之毒性來源。

     This study was aimed to understand the chemical compositions and acute toxicity of smokes generated from five chosen incense coils. Fine original incenses were analyzed for heavy metals with ICP-AES. Elemental analyses on selected elements were also carried out. All SVOCs in the gas-phase of the smoke were collected with XAD-2 resin, and those in the particle-phase with quartz filters. The sampled XAD-2 and quartz filters were then solvent extracted, cleaned up, concentrated and analyzed by GC/MS for PAHs. All concentrated extracts (both original and those cleaned up) were then assayed with Microtox for acute toxicity.
     Total PAH concentrations varied among all five incense coils. In terms of total gas-phase PAHs, Lao Shan had the highest total concentration and Liao the lowest. For particle-phase PAHs, the highest was Zhong Yao and the lowest Liao. The result of elemental analysis show that both TSP and ash had a good correlation between carbon content.
     Results of Microtox show that Chen had the highest acute toxicity while Lao Shan had the lowest toxicity for particle-phase SVOCs. The relative total toxicity (gas + particle phases) of the incense coils is Chen >Hsing Shan >Zhong Yao >Liao >Lao Shan. Also, there was a negative correlation between the sodium content and the toxicity of the gas-phase. In terms of toxicity in gas-phase PAHs, Hsing Shan was the highest and Lao Shan was the lowest; while in the particle phase Zhong Yao was the highest and Lao Shan the lowest.
     The total toxicity of SVOCs is 1~6 times higher than those of PAHs alone, indicating possibly some toxic SVOCs were removed during clean-up process. It is thus recommended that further studies are done in the future to investigate the chemical identities and toxicity of these non-PAH SVOCs.

    總目錄 第一章 前言 1 1.1研究動機 1 1.2 研究目的 2 第二章 文獻回顧 3 2.1拜香之介紹 3 2.1.1拜香之原料組成特徵 3 2.1.2拜香燃煙之粒狀污染物濃度 4 2.1.3香環之製造過程 5 2.1.4拜香燃煙污染防制對策 6 2.2 PAHs之形成機制與來源 9 2.2.1 PAHs之定義 9 2.2.2 PAHs之形成機制 9 2.2.3 PAHs之來源 11 2.3 PAHs之特性 12 2.3.1物理及化學性質 12 2.3.2大氣中PAHs之氣相與粒狀物相分佈 14 2.3.3 PAHs生物毒性 15 2.3.4基因毒性之致癌機制 21 2.4螢光菌之簡介 25 2.4.1螢光菌之生長與發光 25 2.4.2螢光菌之發光機制 25 2.4.3影響螢光菌之發光因素 26 2.4.4螢光菌於毒性試驗上的運用 28 第三章 實驗方法與步驟 30 3.1 樣品選取 30 3.2 實驗架構 31 3.3 採樣 33 3.3.1採樣設計與規劃 33 3.3.2採樣設備 35 3.3.3採樣前之準備 35 3.4 樣品分析鑑定 38 3.4.1 PAHs分析流程 38 3.4.1.1萃取 39 3.4.1.2濃縮與轉溶劑 39 3.4.1.3淨化 40 3.4.1.4再濃縮 41 3.4.1.5氣相層析儀(GC)分析 42 3.4.2元素分析 42 3.4.3重金屬分析 43 3.5 Microtox急毒性測試 48 3.5.1儀器設備 48 3.5.2 Microtox毒性試驗程序 49 3.5.3 EC50值之計算 51 3.6 香環之三成份分析 54 3.6.1測試前處理 54 3.6.2 水份之測定 54 3.6.3 灰份之測定 54 3.6.4 可燃份之測定 55 第四章 數據分析之品質保證及品質控制 56 4.1採樣器流量校正 56 4.2空白試驗 57 4.2.1溶劑空白試驗 57 4.2.2程序空白試驗 57 4.2.3 毒性空白試驗 57 4.3 標準品回收率測試 61 4.4 標準品檢量線之建立 61 4.5 PAHs重複分析 63 4.6 重金屬空白試驗 66 4.6.1溶劑空白試驗 66 4.6.2程序空白試驗 66 4.7 重金屬標準品回收率測試 67 4.8 重金屬標準品檢量線之建立 68 4.9 重金屬重複分析 68 第五章 實驗結果與討論 70 5.1香環燃燒之物理參數 70 5.2 三成份分析結果 72 5.3 香環燃煙中PAHs之含量 73 5.3.1香環燃煙中氣相PAHs之含量 73 5.3.2香環燃煙中固相PAHs之含量 77 5.3.3 TEQ 80 5.4 香環中之元素及金屬含量 81 5.4.1元素分析結果 81 5.4.2重金屬分析結果 82 5.5 Microtox急毒性測試結果 84 5.5.1 SVOCs之毒性測試結果 84 5.5.2 PAHs之毒性測試結果 86 5.5.3 SVOCs與PAHs之毒性比較 87 第六章 結論與建議 89 6.1 結論 89 6.2 建議 90 參考文獻 92

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