本研究將離子對雙親分子（ion pair amphiphile, IPA）hexadecyltrimethylammonium-dodecylsulfate（HTMA-DS）及雙鏈陽離子型界面活性劑（dialkyldimethylammonium bromide, DXDAB）混合，並添加不同莫耳分率的固醇（膽固醇、豆固醇及7-去氫膽固醇），以強制性製程製備出帶正電的陰陽離子液胞（catanionic vesicle）。然後利用粒徑分析儀及傅立葉轉換紅外光譜儀分析液胞的粒徑、界面電位及雙層膜中分子的排列，探討固醇結構對帶正電陰陽離子液胞物理性質的影響。
結果顯示添加固醇會提升液胞的帶電特性，以及調整液胞雙層膜中分子碳氫鏈的排列，而能有效提升液胞的物理穩定性。此外，固醇的添加對液胞雙層膜特性的影響，與固醇結構以及固醇和IPA/DXDAB之間的交互作用有關。相較於膽固醇，豆固醇支鏈上的乙基和雙鍵以及7-去氫膽固醇環狀結構上的雙鍵使製備出之液胞的帶電特性較顯著，液胞雙層膜中分子碳氫鏈則具高流動性且無秩序的排列。固醇結構的效應在等莫耳比例混合之IPA/ditetradecyldimethylammonium bromide（DTDAB）系統中最為顯著，這是由於IPA與DTDAB之間的疏水作用在三種IPA/DXDAB系統中最弱，因此較易顯現固醇結構的效應。值得注意的是，液胞的物理穩定性並未因帶電特性變得較顯著而較佳，因此固醇與IPA/DXDAB之間的交互作用應是決定液胞物理穩定性的主導因素。

In this study, the ion pair amphiphile (IPA), hexadecyltrimethylammonium-dodecylsulfate (HTMA-DS), and double-chained cationic surfactant, dialkyldimethylammonium bromides (DXDAB), were mixed with the addition of various molar fractions of sterols (cholesterol, stigmterol, and 7-dehydrocholesterol) to form positively charged catanionic vesicles by a forced formation process. The size analyzer and Fourier transform infrared spectroscope were then used to analyze the size, zeta potential, and molecular packing in the bilayers of the vesicles to study the effects of sterol structure on physical properties of the positively charged catanionic vesicles.
The results showed that the addition of sterol could enhance the charge characteristic of the vesicles and adjust the packing of the molecule hydrocarbon chains in the vesicular bilayers, resulting in improved physical stability of the vesicles. Furthermore, the effects of added sterols on the vesicular bilayer characteristics were related to the sterol structure and the interaction between sterol and IPA/DXDAB. As compared to cholesterol, the presence of the ethyl group and double bond on the alkyl side chain of stigmterol and a double bond in the sterol ring of 7-dehydrocholesterol led to a pronounced charge characteristic of the vesicles and conformational disorder of the molecule hydrocarbon chains in the vesicular bilayers with high fluidity. The sterol structure effect was more remarkable in the mixed IPA/ditetradecyldimethylammonium bromide (DTDAB) system with a 1：1 molar ratio. This is because the hydrophobic interaction between IPA and DTDAB is the weakest among the three mixed IPA/DXDAB systems, and the sterol structure effect becomes pronounced. It is worth to note that the vesicle physical stability was not improved by the enhanced charge characteristic. Thus, the interaction between sterol and IPA/DXDAB was the dominant factor for determining the vesicle physical stability.

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