此研究利用粒徑及界面電位的分析，探討陰陽離子液胞(catanionic vesicle)與DNA的結合行為。首先以兩種方式製備陰陽離子液胞。第一種是將陰離子型界面活性劑十四烷基硫酸鈉與陽離子型界面活性劑十四烷基三甲基溴化銨，以適當比例在水相中混合，而自發形成帶正電的陰陽離子液胞。第二種是將陰離子型界面活性劑十二烷基硫酸鈉與陽離子型界面活性劑十六烷基三甲基溴化銨於水相中混合，先形成離子對雙親分子（ion pair amphiphile, IPA）的沈澱物，再由IPA透過適當的製程，以強制的方式形成帶正電的陰陽離子液胞。所形成的陰陽離子液胞分散液與不同濃度的type XIV DNA溶液混合，並進行粒徑及界面電位的分析。根據混合前後界面電位的數據，可知帶正電的陰陽離子液胞可與DNA結合。然而，粒徑的分析結果顯示陰陽離子液胞與DNA結合後的穩定性並不理想。因此，在強制製備陰陽離子液胞的配方中添加適量的膽固醇，以便能形成更具穩定性的液胞分散液。分析結果顯示所形成的液胞分散液具有極佳的穩定性，即使經大幅度稀釋後，穩定性仍未受影響。此外，在與plasmid DNA作用後，液胞分散液仍可維持適度的穩定性。

　This study investigated the behavior of catanionic vesicle-DNA association by particle size and zeta potential analyses. Two approaches were applied to prepare catanionic vesicles. The first is to mix the anionic surfactant, sodium tetradecylsulfate, and the cationic surfactant, tetradecyltrimethylammonium bromide, in water with an appropriate ratio, in order to spontaneously form positively charged catanionic vesicles. The second is to obtain the ion pair amphiphile (IPA) first by mixing the anionic surfactant, sodium dodecylsulfate, and the cationic surfactant, hexadecyltrimethylammonium bromide, in water. The IPA was then forced to form catanionic vesicles with positive charges by an appropriate process. The catanionic vesicle dispersions were mixed with various concentrations of type XIV DNA solutions, and the particle size and zeta potential of the mixed systems were analyzed. Based on the zeta potential data obtained before and after mixing, one could conclude that the positively charged catanionic vesicles were able to associate with DNA. However, the particle size measurements indicated that stability of the catanionic vesicles after associating with DNA was unsatisfactory. As a result, a proper amount of cholesterol was added in the formulation of forming catanionic vesicles by the forced approach, in order to prepare vesicle dispersions with enhanced stability. It was found that dispersions of the vesicles in the presence of cholesterol had excellent stability, which was not affected even the dispersions were significantly diluted. Moreover, after interacting with plasmid DNA, the vesicle dispersions still maintained reasonable stability.

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