台灣白花蝴蝶蘭(Phalaenopsis aphrodite subsp. formosana)為台灣原生種，在野外瀕臨絕種的植物，但在蝴蝶蘭育種上為一重要親本。本研究分析台灣白花蝴蝶蘭粒線體的基因組成。藉由抽取白花蝴蝶蘭的花部組織，經由Percoll梯度純化粒線體後，再純化粒線體DNA，利用Roche 454 GS junior pyrosequencing方式定序建構的shot-gun和8 kb mate-paired基因庫，並利用Illumina方式定序建構的shot-gun和5 kb mate-paired基因庫，而後將由這些基因庫定序出的片段(reads)進一步組裝成更長的序列，總共得到34條粒線體DNA序列，總長為682 kb，GC含量為44.7%、重複性序列佔總長的34.35%、轉座子佔5.36%。並註解出39個蛋白質編碼基因、3個rRNA、14個tRNA。蛋白質編碼基因佔mtDNA總長的6.9%，粒線體DNA含有來自葉綠體及細胞核DNA的序列，其分別佔2.1%與0.8%。利用單子葉植物與阿拉伯芥的粒線體基因組共同擁有的26個基因畫出演化親緣關係圖顯示出，白花蝴蝶蘭與大部分的禾本科植物可形成一群。

The moth orchid, P. aphrodite subsp. formosana is the endemic species in Taiwan. The mitochondrial DNA (mtDNA) was isolated from the floral tissues of moth orchid by percoll gradient. Subsequently, the mtDNA was sequenced through next generation sequencing (NGS). Up to date, 34 potentially mtDNA containing contigs accounting for approximately 682 kb in length were obtained. The GC content of mtDNA is 44.7%. By BLAST mtDNA to NCBI database, 39 protein coding genes, 3 rRNA and 14 tRNA genes specific to mitochondria were annotated. In addition, phylogenetic analysis based on the concatenation of 26 common mitochondrial protein coding genes among monocots and Arabidopsis, the moth orchid could be clustered with most of plants in grass family.

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