本論文之乾式檢測試紙系統應用於菜鴨之tmigd1基因之單核苷酸多型性(Single Nucleotide Polymorphism)檢驗，為先前研究初步測試開發可行之試紙檢測系統之延續研究，其免疫層析試紙主要藉由修飾核酸探針之奈米金粒子作為顯色單元，並使用鏈親和素(Streptavidin)作為試紙測試帶之探針，捕抓Primer Extension (PEXT)反應中對應SNP位置被延長而引入Biotin-16-dUTP之產物。
本論文針對試紙的特性研究分為兩個部份，分別為再現性測試與試紙參數最佳化實驗。在再現性測試實驗中，使用6組萃取自不同鴨隻之DNA樣品進行SNP檢測。其測試結果顯示出G/G基因型之試紙訊號特異性較A/A基因型為佳，且由肉眼觀察到每組試紙皆有明顯之假陽性(False Positive)訊號的產生。而在不添加PEXT產物之試紙測試中，發現到非特異性吸附之假陽性訊號的出現。
因此在試紙參數最佳化實驗，藉由調整試紙參數最佳化的測試來完全消除非特異性吸附之訊號。試紙的吸附強度訊號會受到試紙中參數的影響。故此，針對硝化纖維薄膜的孔徑(Pore Size)大小、奈米金粒子的用量以及Streptavidin的施加量進行調控，同時結合影像分析軟體ImageJ對吸附訊號進行強度的計算，得到完全消除非特異性吸附訊號之試紙參數。

最後，使用前述實驗所獲得之6組無非特異性吸附訊號之試紙參數，再使用一組G/G基因型與一組A/A基因型DNA樣品進行再現性測試。最終獲得最佳化之參數為15 μm的孔徑薄膜，奈米金用量為3 μL以及0.08 μg的Streptavidin施加量，完整的檢測時間5分鐘，即可得到偵測訊號結果。

In this research, we did the reproducibility test of dry-reagent strip biosensor for SNP detection in tmigd1 gene of Tsaiya duck (Anas platyrhynchos), and optimized the parameters of the strip.
The SNP detection system combined primer extension (PEXT) method with the lateral flow immunoassay for SNP discrimination. To validate the reproducibility of dry-reagent strip, we conducted the tests with 6 DNA samples. According to our results, the signal specificity of the strip detection was better for G/G genotype than A/A genotype samples. In addition, we found the appearance of false positive signal in every strip test results, and even affected the interpretation of the SNP detection results by naked eyes.
Therefore, we tried to control the pore size of the nitrocellulose membrane, gold nanoparticle usage and loading amount of streptavidin to eliminating the false positive signal without the addition of PEXT product. We got 6 sets of parameters of the strip, and then applied these strips to SNP detection. According to the detection results, we obtained the optimal strip parameter set for using 15 μm pore size of the membrane, 3 μL usage of gold nanoparticle and 0.08 μg loading amount of streptavidin.

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