本研究成功開發出具有高線性度、高靈敏度及穩定度之桌上型快速免疫分析儀，利用免疫呈色反應結果作為檢測標的，讓免疫呈色反應偵測於定量分析上更加精確。開發之免疫分析儀包含硬體及軟體兩部分，硬體方面設計出一台判讀系統外殼主體，裝載Raspberry Pi Zero W開發板、Universal Serial Bus (USB)攝影機、LED光源、LCD觸控式液晶顯示、RTC模組(Real-Time Clock)、導光板及光學暗房，並製作出卡匣載台，為校正卡匣放置區、快速響應矩陣碼卡匣放置區及檢測卡匣放置區。軟體部分使用Python整合模組OpenCV、NumPy、Matplotlib、SQLite及Bluetooth撰寫出偏光校正、QR code解碼、影像處理、資料庫儲存、藍芽傳輸與使用者介面之程式。由實驗結果可發現，本研究所開發之桌上型快速免疫分析儀應用於糞便潛血之檢測試劑量測下，正確讀取使用者輸入之Control、Test線(C、T線)位置，並建立出高線性度之濃度與灰階值擬合曲線，作為量化分析依據，在糞便潛血濃度偵測下，該曲線之R square值可達0.9888並且在重複實驗下仍可保持良好的精準度(變異係數<20 %)。

This thesis presents a highly sensitive and stable desktop rapid immunoassay instrument for fecal occult blood (FOB). The inspection is based on immunochromatographic assay which is widely applied to fecal occult blood detection. Combined with the immunochromatographic strip, the detection results obtained by desktop rapid immunoassay instrument showed good correlation with the concentration of FOB. The system is composed of hardware and software parts and the layout of reader was designed by SolidWorks. The analyzing programs including a main program, polarization calibration, QR code, image processing and bluetooth transmission were compiled by Python. After verification of each parts of the system, we found that the desktop rapid immunoassay instrument successfully detected fecal occult blood reagent from 0 ng/mL to 450 ng/mL.

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