本研究利用動態離心電紡絲技術 (Centrifuged-Electrospinning Methodology)，製備聚丙烯腈纖維(PAN)，並經由1200℃-1500℃的碳化程序製得奈米碳纖維(CNFs)。透過磁場之誘導，於奈米碳纖維固定化成長針狀鎳觸媒(Needle-like Nickel)，製成碳纖維/針狀鎳(CNFs-Ni)觸媒感測器材料，並應用於葡萄糖、甲醇、甲醛之非酵素型電流式感測器(Amperometric non-enzymatic sensors)。CNFs經由拉曼光譜(Raman)儀和四點碳針(four-point probe)分析結果顯示，當燒結溫度到達1500℃，其ID/IG比值達0.74且導電度為18.03 Scm-1，證實經由外加離心場之誘導，可使CNFs導電度上升。使用化學分析電子光譜儀(ESCA)、XRD以及掃描式電子顯微鏡(SEM)分析CNFs-Ni表面元素組成及型態結果顯示，CNFs結構上的π-π*鍵結組成百分比隨著燒結溫度上升，針狀鎳均勻的固定化於CNFs的表面。進一步地，藉由電化學分析感測器之性能顯示，在溫度燒結到1400℃ (CNFs1400-Ni)具有最高之靈敏度；其中，在葡萄糖感測的靈敏度(Sensitivity)為6703 μA mM-1cm-2、線性範圍(Linear range)為0.021 mM-0.6 mM (R2=0.9935)、應答時間(Response time)為10秒、再現性(Reproducibility)之相對標準差(Relative standard deviation)為6.97%、穩定度(Stability)為16.94%之偏差值；在甲醇方面，靈敏度為610 μA mM-1cm-2，線性範圍為0.245 mM-1.5 mM (R2=0.9979) ，應答時間為6秒，再現性之相對標準差為4.27%，穩定度為24.13%之偏差值；在甲醛檢測方面，靈敏度為2306 μA mM-1cm-2、線性範圍為0.059 mM-1.5 mM (R2=0.9918)、應答時間為6秒、再現性之相對標準差為8.81%、及穩定度為15.75%之偏差值。另一方面，研究自製電流式氣相感測器(Amperometric gas sensor)來偵測空氣中的甲醛濃度，透過PTFE防水透氣膜，使氣相甲醛擴散進入感測器之中，也使感測器中之電解質NaOH不會洩漏。而電極方面則採用低鎳濃度製備CNFs-Ni 1-8。經由SEM及XRD鑑定，表面針狀鎳型態並無完整包覆住CNFs，這使得溶液內之活性物質能更迅速地進入電極內部，使應答時間大幅下降，也使得電極被充分應用，靈敏度提升。電化學分析結果顯示，CNFs-Ni 1-8其氣相甲醛靈敏度為1.51 μA ppm-1、線性範圍為0.5 ppm-10 ppm(R2=0.9984)、應答時間為5秒；穩定度方面，經過9小時連續測試電流仍無明顯下降趨勢。此結果顯示與其他文獻相比，為具有商業化競爭力之電流式氣相感測器。

The preparation of needle-like nickel immobilized on centrifuged-electrospinning carbon nanofibers (CNFs-Ni) via electroless plating and their applications in amperometric non-enzymatic sensors are described. Poly acrylonitrile (PAN) nanofibers are prepared by centrifuged-electrospinning methodology which can escalate electrical conductivity of carbon nanofibers (CNFs). PAN nanofibers are carbonized in various temperature (1200 ℃-1500 ℃). CNFs are confirmed by scanning electron microscopy (SEM), electron spectroscopy for chemical analysis (ESCA), Raman spectroscopy and four-point probe. CNFs-Ni are analyzed by SEM, X-ray diffraction (XRD). Under optimal conditions (CNFs1400-Ni), the glucose sensor shows high sensitivity (6703 μA mM-1cm-2), linear range from 0.021 mM to 0.6 mM (R2=0.9935) and fast response time (10 s). As compared to other commercial substrates, the advanced CNFs-Ni electrode exhibits high electrocatalytic activity and good response.

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