近年來人工牙根的植入越來越普及，但伴隨著術前計畫不夠完善、患者口腔衛生習慣不佳等原因，仍可能導致牙根周圍炎的情況發生。近年來因牙根植體形狀逐漸多樣化、抗生素的濫用及細菌耐藥性增加，令傳統機械方式與抗生素之治療成效日漸不彰。為能達到有效的殺菌效果，光動力療法 (Photodynamic therapy, PDT) 已逐漸成為新興的牙根周圍炎治療方式。本研究主要分為材料表面之抗菌試驗及光動力療法。首先在抗菌試驗部分，分別製備P、SB、SLA、MAO和5SrMAO等五種不同表面處理之鈦基材，並於試片上培養Escherichia coli (E. coli) 與 Porphyromonas gingivalis (P. gingivalis)，探討不同表面條件下材料之抗菌能力。同時針對光動力療法於懸浮培養及不同材料表面之殺菌效果進行研究，並觀察光動力療法對細胞毒性之影響。由SEM與Optical density實驗結果發現，低的表面自由能、粗糙度以及含有鍶元素之材料表面具有較少的細菌貼附量。而經過光動力療法實驗後，發現不論在懸浮培養以及試片培養上，E. coli與P. gingivalis之存活率明顯下降至60 % 與20 %，且不論在平坦或粗糙的試片上，皆可達到相同之殺菌效果。綜合研究結果，顯示光動力療法在各表面形態下皆有一致的殺菌效果，同時也能有效的對細菌造成毒殺作用，故未來在牙科臨床上，將有其參考價值。

There is a growing popularity of dental implants in the market. With the poor oral hygiene, imperfect plan of pre-operation, patient with smoking or periodontal disease may lead to peri-implantitis. With the variety of screw shape and resistance-related bacteria, photodynamic therapy (PDT) becomes a new treatment for peri-implantitis.
In this study, two types of Gram-negative bacteria would be chose, including Escherichia coli (E. coli) and Porphyromonas gingivalis (P. gingivalis). These bacterial were be used to research the anti-bacterial properties and combined with Toluidine Blue O (TBO) to perform photodynamic therapy. First, the titanium discs with different surface morphology would be prepared such as Polish, SB, SLA, MAO, and 5SrMAO to discuss the anti-bacterial properties. Then, we used the PDT to study the bactericidal effect of suspension, biofilm system and cytotoxicity of cells.
From the SEM and optical density results showed that with low surface-free energy, low surface roughness, and surface containing strontium ion would reduce plaque accumulation in our anti-bacterial tests. In the PDT experiments, the results indicated that the survival rate of bacteria would decrease after PDT no matter on E. coli or P. gingivalis system and suspension or biofilm system. Then, PDT showed no significant difference between each groups of titanium discs. In sum, this study can conclude that strontium ion can inhibit the growth of bacterial and PDT will become a potential treatment for peri-implantitis in the future.

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