摘要
組織工程的目的為：利用多孔性的支架（scaffold）來模擬人體原有的extracellular matrix，使細胞附著、增生、遷移，然後產生功能，以重建缺損的組織或器官。氫氧基磷灰石（HA）具有良好的生物相容性與生物活性，植入人體後不會引起毒性，不刺激組織，具有引導骨骼相內生長，及在骨骼外組織誘導骨細胞分化生長的能力。利用磷酸鈣鹽泥(Calcium phosphate ceramic slurry)製成的多孔性HA磷酸鈣鹽具有適當的強度，能控制其孔洞大小、孔隙度，可消毒，也具有生物可吸收性，磷酸鈣鹽泥的可塑性並能製出3-D不規則的立體結構，是一種適合作為組織工程支架的材料。
本研究將磷酸鈣鹽泥混合NaCl粉末填模加壓成型，利用salt leaching方式製成多孔性HA磷酸鈣鹽，並改變不同製程條件來同時提高材料的抗壓強度與孔隙度，同時以SEM觀察材料表面形態，並以XRD分析不同製程條件與不同添加物含量對磷酸鈣鹽組成的影響，最後進行PH值量測及細胞測試，期望能獲得一個適合應用於組織工程的scaffold。
實驗發現磷酸鈣鹽泥中NaCl的含量增加，能提高磷酸鈣鹽的孔隙度，但卻使抗壓強度降低。加長磷酸鈣鹽泥的浸泡時間，或利用適當條件的熱處理能將磷酸鈣鹽的強度提高，同時不影響材料孔隙度與結晶性差的apatite相。細胞測試發現，當磷酸鈣鹽孔隙度高，細胞容易附著，細胞毒性也低。因此利用磷酸鈣鹽泥混合水溶性鹽類，浸泡後加上適當熱處理，利用磷酸鈣鹽泥中原本就有的微孔洞加上salt leaching產成的巨孔洞，形成一巨孔微孔同時存在的高孔隙度結構，加速磷酸鈣鹽吸收與新骨生長並具有一定的強度，非常有潛力成為軟骨/硬骨重建的組織工程支架。

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