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研究生： 王致凱
Wang, Zhi-Kai
論文名稱： 雙輸出幾丁聚醣電紡奈米纖維及其電漿合成表面固定的銀奈米粒子
Dual-Supplied Electrospinning of Chitosan Nanofibers with Plasma-Synthesized and Surface-Immobilized Silver Nanoparticles
指導教授： 郭昌恕
Kuo, Chang-Shu
學位類別： 碩士
Master
系所名稱： 工學院 - 材料科學及工程學系
Department of Materials Science and Engineering
論文出版年： 2015
畢業學年度： 104
語文別： 英文
論文頁數： 70
中文關鍵詞： 電紡絲 、奈米纖維 、幾丁聚醣 、電漿處理 、銀奈米顆粒 、抗菌測試
外文關鍵詞： electrospinning, nanofibers, chitosan, plasma treatment, silver nanoparticles, antibacterial test
相關次數： 點閱：171  下載：0 
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    • 本研究使用兩種不同雙軸輸出電紡絲製程製備電漿還原銀奈米顆粒固定於表面的幾丁聚醣奈米纖維。首先，雙輸出單軸電紡絲製程是將幾丁聚醣與交聯劑兩溶液分別配置，兩溶液在從針頭擠出之前相互混和的電紡絲製程。第二種雙輸出同軸電紡絲製程使用實驗室製作的同軸針頭，內針頭輸入幾丁聚醣溶液，外針頭輸入聚氧化乙烯、交聯劑和銀前驅物。本實驗謹慎地研究電紡絲製程，結果指出，經過氬電漿處理轟擊後，幾丁聚醣奈米纖維直徑約為135奈米，銀前驅物和硝酸銀還原成銀奈米顆粒並固定於幾丁聚醣纖維表面。抗水性測試結果顯示幾丁聚醣奈米纖維有良好機械性質，銀奈米顆粒也穩固的在奈米纖維的表面，接下來在抗菌測試是以過濾的方法研究，電紡奈米纖維抗菌過濾的結果顯示，纖維表面固定銀奈米顆粒數量與抗菌性呈正比關係。

    Plasma-synthesized silver nanoparticles (Ag NPs) were immobilized on the surface of chitosan-based nanofibers fabricated by two kinds of dual-supplied electrospinning processes. In the first fabrication approach, the dual-supplied electrospinning was conducted by two flows that carried the chitosan and the crosslinking agent separately. The two flows were mixed right before the ejection of the electrospinning solutions. The second approach involved the use of a lab-made co-axial nozzle that constructed the chitosan nanofiber cores and the poly(ethylene oxide) (PEO) outer sheath which also carried the crosslinking agent and the silver precursor. Electrospinning processes were carefully investigated in this research project. The resulting chitosan nanofibers with an average fiber diameter as small as about 135 nm were then treated with the argon plasma bombardment. Silver precursors, the silver nitrate, within the chitosan nanofibers were converted to metallic Ag NPs, which were immobilized on the surface of these nanofibers. Water resistance confirmed these robust chitosan nanofibers, as well as the stabilities of surface-immobilized Ag NPs. Following antibacterial tests based on the water filtration apparatus were investigated. And the antibacterial performance of these electrospun chitosan nanofibers were proportionally enhanced by the population of the surface-immobilized Ag NPs.

    Abstract I 摘要 II 致謝 III Table of contents IV List of illustration VII List of table X Chapter 1 Introduction 1 1.1 Introduction of Nanofibers and Electrospinning 1 1.2 Chitosan and Electrospun Chitosan Nanofibers 3 1.3 Coaxial Electrospinning 6 1.4 Ag Nanocomposites 7 1.4.1 Preparation of Ag nanocomposites 8 1.4.2 Ag nanoparticles as antimicrobial agent. 9 1.5 Plasma 9 1.5.1 Nonthermal plasma for surface modification of polymers 11 1.5.2 Nonthermal plasma sources 12 1.6 Electrospun Nanofibers for Filtration 15 1.6.1Electrospun nanofibers for air filtration 15 1.6.2 Electrospun nanofibers for water filtration 16 Chapter 2 Motivation 17 Chapter 3 Experiment Design 19 3.1 Materials and Experimental Instruments 19 3.2 Solution Preparation of Dual Supplied Electrospinning 19 3.2.1 CS-PEO solution 19 3.2.2 TPP solution 20 3.2.3 CS-PEO core solution 20 3.2.4 PEO-TPP-Ag shell solution 20 3.3 Fabrication of Coaxial Spinneret 20 3.2 Single Supplied Electrospinning 21 3.3 Dual Supplied Electrospinning 22 3.3.1 Dual supplied: single electrospinning 22 3.3.2 Dual supplied: coaxial electrospinning 23 3.4 Plasma Treatment for Synthesize Ag Nanoparticles 25 3.5 Nanofibers Characterization 26 3.5.1 Scanning electron microscopy (SEM) 26 3.5.2 Transmission electron microscope (TEM) 27 3.5.3 UV- Visible spectrophotometer 27 3.5.4 Fourier transform infrared spectroscopy (FT-IR) analysis 28 3.5.5 X-ray photoelectron spectroscopy (XPS) 28 3.5.6 Antibacterial test 29 Chapter 4 Result and Discussion 34 4.1 Conventional Single Electrospinning 34 4.2 Dual Supplied Electropinning 36 4.2.1 Dual supplied single electropinning 36 4.2.2 Dual supplied coaxial electrospinning 38 4.3 Plasma Treatment on Nanofibers 45 4.3.1 Plasma-synthesized Ag nanoparticles on CS-PEO-TPP-Ag nanofibers 45 4.3.2 The effect of plasma etching 47 4.3.3 Core-shell nanofibers diameter distribution 50 4.3.4 Plasma chamber investigation 52 4.3.5 FTIR analysis 55 4.3.6 XPS analysis 57 4.3.7 TEM analysis 60 4.4 Antibacterial Test 63 Chapter 5 Conclusion 65 Chapter 6 Reference 66

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