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研究生： 李家瑋
Lee, Chia-Wei
論文名稱： γ-Fe2O3奈米粒子的表面修飾及其生化上的應用
Surface modification of γ-Fe2O3 nanoparticles and its bio-application
指導教授： 葉晨聖
Yeh, Chen-Sheng
學位類別： 碩士
Master
系所名稱： 理學院 - 化學系
Department of Chemistry
論文出版年： 2004
畢業學年度： 92
語文別： 中文
論文頁數： 68
中文關鍵詞： 生化應用 、表面修飾 、三氧化二鐵奈米粒子
外文關鍵詞： bio-application, surface modification, γ-Fe2O3 nanoparticle
相關次數： 點閱：81  下載：8 
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    • 　　一些具有磁性的金屬氧化物奈米粒子，如三氧化二鐵及四氧化三鐵，已經被廣泛的研究並發展在感測、核磁共振造影、藥物傳遞以及各種化學及生物物質的分離…等。目前有很多的研究正著手於用各種大分子來修飾磁性粒子的表面，如DNA或生物巨分子。
    　　利用熱分解的合成方法，我們已經成功地合成分散的γ-Fe2O3奈米粒子，粒徑分佈大約在8.4±1.4 nm。其表面修飾的是長碳鏈的保護劑，我們可以有效的將其表面修飾上銨鹽，並且可以分散於水溶液中。
    　　氧化鐵粒子經過銨鹽修飾之後，我們利用其表面性質，可以將一個雙官能基的交連結試劑修飾到氧化鐵粒子的表面，為此交連結試劑分子的一端，可以與-NH2基形成amide鍵；而另一端將與硫醇基形成-C-S-鍵。
    　　在經過此分子的修飾之後，磁性的氧化鐵粒子可以與硫
    醇化的寡聚核苷酸產生連結。最後，可得到磁性粒子與寡聚核苷酸的複合物。
    　　由於去氧核糖核酸具有特殊的專性，所以修飾上寡聚核苷酸的磁性奈米粒子可以用來做很多生化上的應用。
    　　利用三股序列的雜交反應，我們可以成功地將個別修飾上寡聚核苷酸的金與氧化鐵奈米粒子形成複合物。經由雜交所形成的複合物(Au/Fe2O3)，在加熱至70℃的溫度下，會破壞氫鍵，使複合物(Au/Fe2O3)分開，而回溶於水溶液中。這也提供了證據證明接在氧化鐵磁性粒子上的寡聚核苷酸仍然具有雜交的能力，而可利用於生化上面的應用。

    　　Magnetically active nanoparticles, such as Fe2O3 and Fe3O4, have been extensively investigated because of their application in sensing, imaging, delivery and separation of various chemical and biological entities. Considerable efforts have been devoted to modify the surfaces of magnetic particles using macromolecules, such as DNA and polymers. Following the thermodecomposition synthetic route, we have successfully prepared well dispersedγ-Fe2O3 nanoparticles. The fabricated Fe2O3 nanoparticles with the average size of 8.4 ± 1.4 nm can be effectively transferred into aqueous medium using an ammonium salt and can be dispersed in aqueous solution.
    　　Based on the properties of the particle surfaces, the magnetic particles were modified with a bifunctional linker, where one terminal would react with amine group to form an amide bond while the other side would form a -C–S- bond. After the modification of the linker, magnetic nanoparticles can immediately catch thiol modified oligonucleotides through the formation of –C-S- bond. Once, the magnetic particles were conjugated with oligonucleotides. They were investigated by both electrophoresis and fluorescence measurements.
    　　Since DNA has highly specific base-pairing nature, the developed magnetic-oligonucleotide conjugates could be used in a variety of biological applications. Using a three-strand hybridization system, we were able to conjugate oligonucleotides modified Fe2O3 and Au nanoparticles. The resulting hybridization conjugates (Fe2O3/Au) could be redispersed after heating to 70℃ through dehybridization process.

    中文摘要.........................................I 英文摘要........................................II 表目錄.........................................III 圖目錄..........................................IV 第一章緒論.......................................1 1.1 奈米科技.....................................1 1.2 奈米粒子的性質...............................4 1.2.1 表面效應...................................4 1.2.2 小尺寸效應(體積效應) ......................5 1.2.3 量子尺寸效應...............................5 1.3 奈米粒子的製備...............................9 1.3.1 物理方法(Physical Method)..................9 1.3.2 化學方法(Chemical Method)..................12 1.4 影響奈米粒子穩定度的因素.....................16 1.5 金屬奈米粒子的表面電漿共振...................23 (surface plasmon resonance) 1.6 生物分子(DNA)的介紹..........................29 1.7 磁性奈米粒子的簡介...........................30 1.8 磁性理論.....................................32 第二章水溶性磁性奈米粒子(γ-Fe2O3)的製備.........38 2.1 研究動機與目的...............................39 2.2 實驗藥品與儀器...............................40 2.2.1 藥品.......................................40 2.2.2 儀器.......................................40 2.3 實驗步驟.....................................42 2.3.1 製備油性三氧化二鐵(γ-Fe2O3)磁性奈米粒子...42 2.3.2 製備水溶性三氧化二鐵(γ-Fe2O3)磁性奈米粒子.42 2.4 結果與討論...................................43 2.4.1 TEM分析....................................43 2.4.2 UV-Vis 分析................................43 2.4.3 XPS 分析...................................43 2.4.4 SQUID 分析.................................44 2.5 結論.........................................45 第三章磁性奈米粒子與生物分子的結合...............50 3.1 研究動機與目的...............................51 3.2 實驗藥品與儀器...............................51 3.2.1 藥品.......................................51 3.2.2 儀器.......................................52 3.3 實驗步驟.....................................53 3.3.1 製備水溶性磁性奈米粒子.....................53 3.3.2 將GMBS 分子修飾在氧化鐵奈米粒子表面........53 3.3.3 將硫化寡聚核苷酸修飾在氧化鐵表面...........53 3.4 結果與討論...................................55 3.4.1. TEM 分析..................................55 3.4.2. XPS 分析..................................55 3.4.3. 螢光顯微鏡分析............................55 3.4.4. 電泳分析..................................55 3.4.5. 雜交反應..................................56 3.4.6. 加熱反應..................................57 3.5 結論.........................................58 參考文獻.........................................65

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