人體尿蛋白在腎炎與腎病變診斷扮演重要的角色，尿蛋白的自我檢測可以達到預防勝於治療效果。本研究所製備電化學尿蛋白感測器，以牛血清白蛋白(bovine serum albumin, BSA)為待測物，利用循環伏安法(CV)探討不同濃度之白蛋白溶液的峰電流。
在2,2'-聯氮-雙(3-乙基苯并噻唑啉-6-磺酸) (2,2’-azino-bis(3-ethyl-
benzothiazoline-6-sulfonic acid), ABTS)分別與兩種不同碳材混摻所成的修飾電極，並將其浸泡於溫度25oC、pH 6.5的白蛋白緩衝溶液中1小時後使用循環伏安法分析。結果顯示ABTS混摻多壁奈米碳管的靈敏度(0.520 μA/ppm)為ABTS混摻碳黑(0.180 μA/ppm)的2.89倍，而且比較不受背景電流干擾；感測濃度範圍皆為0-200 ppm。
在上述條件之下，多壁奈米碳管混摻ABTS為修飾電極的電化學反應為吸附控制。吸附時的攪拌靈敏度可以從0.520 μA/ppm提高到2.633 μA/ppm。在無攪拌的系統中，溶液溫度從25oC改變到35oC，靈敏度從0.520 μA/ppm 增加至1.533 μA/ppm。
多壁奈米碳管混摻ABTS為修飾電極含浸在溫度25oC的白蛋白緩衝溶液中1小時，隨著電解液的pH由4增加到6.5、8與10時，靈敏度由0.196 μA/ppm改變到0.520 μA/ppm、0.463 μA/ppm與0.044 μA/ppm。

To fabricate a modified electrode, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) was blended with one of two carbon materials: multi-wall carbon nanotube (MWCNT) and carbon black (XC-72). According to the experiment results, when the modified electrodes were soaked in a BSA solution of pH 6.5 at 25oC for one hour, the sensitivity of ABTS/MWCNT modified electrode (0.520 μA/ppm) was 2.89 times as much as that of ABTS/XC-72 modified electrode (0.180 μA/ppm). Their detection range was the same between 0 and 200 ppm. Under the above condition, the electrochemical reaction of BSA was controlled by the adsorption on the surface of ABTS/MWCNT modified electrode. During adsorption period, agitation increased the sensitivity from 0.520 to 2.633 μA/ppm. Sensitivity increased from 0.520 to 1.533 μA/ppm when electrolyte temperature increased from 25oC to 35oC. As the pH increased from 4, the sensitivity of the ABTS/MWCNT modified electrode increased from 0.196 μA/ppm to 0.520 μA/ppm at pH 6.5, then decreased to 0.044 μA/ppm at pH 10, after soaking in the BSA solution at 25oC for one hour.

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