本研究將陽離子型界面活性劑hexadecyltrimethylammonium bromide（HTMAB）與陰離子型界面活性劑sodium dodecylsulfate （SDS）於水相中混合，以製備出離子對雙親分子（ion pair amphiphile）hexadecyltrimethylammonium-dodecylsulfate（HTMA-DS）。然後以HTMA-DS為主材料分別添加不同比率的陽離子型界面活性劑HTMAB及雙碳氫鏈二甲基溴化銨（DXDAB），透過適當製程製備出帶正電的陰陽離子液胞。當添加的陽離子型界面活性劑莫耳分率Xcationic為0.5時，液胞的粒徑穩定性最好，液胞界面電位則不隨添加之陽離子型界面活性劑的種類及比率而有明顯改變。因此液胞的粒徑穩定性除了與液胞之間的電荷斥力有關外，還必須考慮雙層膜結構的緊密性及排列缺陷。
　　此外，針對Xcationic為0.5時所形成的HTMA-DS／DXDAB液胞，進一步探討白蛋白的存在對液胞物理穩定性的影響。實驗結果顯示白蛋白的吸附會降低HTMA-DS／DXDAB所形成之陰陽離子液胞的界面電位，甚至在較高濃度白蛋白的情況下還出現液胞電性反轉的現象。在液胞電性反轉之前，液胞的粒徑穩定性隨界面電位的值變小而變差。然而當液胞電性反轉後，因白蛋白吸附而帶有負電之液胞的穩定性反而提升。在這些液胞系統中，似乎又以含碳氫鏈較短之雙十四烷基二甲基溴化銨的液胞雙層膜結構較為鬆散，受到白蛋白的影響也較大。本研究也嘗試利用膽固醇的添加來促進液胞的物理穩定性，發現在不同的HTMA-DS／雙十八烷基二甲基溴化銨混合比例下，膽固醇的加入可提升液胞的粒徑穩定性及界面電位。在白蛋白的存在下，膽固醇的添加及降低雙十八烷基二甲基溴化銨的含量，則可有效降低白蛋白對HTMA-DS液胞之穩定性所造成的影響。

　　In this study, the ion pair amphiphile, hexadecyltrimethylammonium-dodecylsulfate (HTMA-DS), was obtained as precipitate by mixing aqueous solutions of the cationic surfactant, hexadecyltrimethylammonium bromide (HTMAB), and the anionic surfactant, sodium dodecylsulfate (SDS). The HTMA-DS was then mixed with HTMAB and dialkyldimethylammonium bromide (DXDAB), respectively to form positively charged catanionic vesicles by a proper process. The highest physical stability of the catanionic vesicles was found at the molar ratio of the cationic surfactant Xcationic＝0.5. However, the zeta potential of the catanionic vesicles was not significantly affected by the type and molar ratio of the added cationic surfactants. It appeared that the physical stability of the vesicles was dependent not only on the electrostatic repulsion between vesicles, but also on the packing and arrangement defects of the bilayer structures.
　　Moreover, the physical stability of the HTMA-DS/DXDAB vesicles with Xcationic＝0.5 in the presence of bovine serum albumin (BSA) was investigated. The experimental results indicated that the adsorption of BSA on the vesicles could decrease the zeta potential of the catanionic vesicles. With higher BSA concentrations, charge reversal of the vesicles might be observed. Without the charge reversal of the vesicles, the vesicle size stability became lower when the zeta potential was decreased. Nevertheless, with the charge reversal of the vesicles, the stability of negatively charged vesicles with BSA adsorption became higher. Among the HTMA-DS/DXDAB vesicle systems, the bilayer structures of the HTMA-DS/ditetradecyldimethylammonium bromide vesicles seemed to be less compact and were more affected by BSA. The addition of cholesterol to improve the vesicle physical stability was also tested, and it was found that the presence of cholesterol in the vesicles of HTMA-DS/dioctadecyldimethylammonium bromide (DODAB) with various mixing ratios could enhance the size stability and zeta potential of the vesicles. In the presence of BSA, the addition of cholesterol and a lower content of DODAB in the vesicles could effectively reduce the BSA influence on the physical stability of the vesicles.

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