本研究分別將萃取自破囊壺菌之兩種不同批次的微藻油添加不同莫耳比率的維他命E醋酸酯製備液胞，分析其粒徑、界面電位及穩定天數，並利用熱差式掃描卡量計法、螢光偏極化法及傅利葉轉換紅外光譜法分析液胞雙層膜的膜流動性及相轉移行為，以及利用Cell Counting Kit-8法測試藻油液胞對人類正常支氣管上表皮細胞的細胞毒性。此外，以穩定之液胞系統進行一連串分離和濃度測定，了解不同的脂肪酸碳鏈結構對於形成之帶負電藻油液胞物化特性及包覆效率的影響。
兩種批次藻油皆可以形成穩定性佳的帶負電液胞，對生物具低毒性，在低濃度中皆保持80%以上的細胞存活率。兩種批次藻油形成液胞之初始粒徑和界面電位隨脂肪酸碳鏈分布而不同。添加維他命E醋酸酯後，發現批次一藻油液胞因原雙層膜排列較整齊而膜結構變化不大，批次二藻油因原雙層膜排列缺陷較多而膜結構變化較顯著。
由兩種批次藻油製備之液胞包覆維他命E醋酸酯的結果，可以發現脂肪酸結構差異對包覆效率的影響並不顯著，主要影響包覆效率的因素為材料多寡，即使批次一藻油液胞的流動性較低，在高的維他命E醋酸酯濃度下也有較高的包覆效率。

Microalgal lipid is the material with high utilization value which can act as low cost and high nutritional value delivery carrier. In this study, a microalgal lipid mixture rich in DHA was extracted from an isolated Thraustochytrium sp. DJ3 strain. In order to investigate the encapsulation capability of the microalgal lipid as hydrophobic materials carriers, this study aims to apply the microalgal lipid as delivery carrier of vitamin E acetate. The microalgal lipid was used to fabricate bilayered vesicles with negative charges by a forced formation approach. Moreover, the vesicles were used as carriers for encapsulating vitamin E acetate. Physical properties of the vesicles, including initial size and zeta potential, were measured. Fluidity of the vesicular membranes with and without vitamin E acetate was then investigated by IR spectroscopy, fluorescence polarization and differential scanning calorimetry. Cytotoxicity of microalgal lipid vesicles was evaluated by WST-8 cell viability assay. The results showed that stable vesicles with negative charges could be successfully formed from the microalgal lipid, remaining stable after being diluted to 0.026 wt%, whose original concentration is 2.6 wt%. The cell viabilities of BEAS-2B cell treated by the microalgal lipid vesicles are close to 100% while the concentration is enough low. The vitamin E acetate entered the bilayer and affected the initial size and zeta potential of the vesicles, lowering the membrane fluidity of the vesicles. The existence of vitamin E acetate had no adverse effect on the stability of the microalgal lipid vesicles and presented the high encapsulation efficiency over 80 mol%, which showed the potential of using microalgal lipid for fabricating vitamin E acetate-encapsulating vesicles.

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