天然有機物(Natural Organic Matter)是大量存在於河川、湖泊、水庫等飲用水水源中之複雜有機物混和物，對水質及水處理程序有重大影響，其中對人體健康之重大影響是天然有機物於消毒程序中，會與消毒劑反應生成具有致癌性的消毒副產物，其中以三鹵甲烷(THM)與鹵化乙酸(HAA)占有較高的比例。
本研究以腐植酸(Humic acid)、黃酸(Fulvic acid)、蛋白質(BSA)與藻酸(Alginic acid)等四種有機物，模擬天然原水中由土壤、植物殘體降解產生的腐植質，以及水生動物、藻類的代謝物質。作為疏水性與親水性有機物代表。並以高效能粒徑排除層析儀(HPSEC)連接線上UV/Vis偵測器與OC偵測器分析個別有機物與混合有機物之分子量分佈與特性。搭配Peak fit軟體分析HPSEC圖譜中各有機物族群面積之變化。之後與消毒副產物生成潛勢之結果做比較。
研究結果顯示，腐植酸和黃酸等腐植質類的有機物，因含有較多的苯環和酚基，具有相當高的消毒副產物生成潛能。而大分子的蛋白質，也對THM與HAA有很高的貢獻度。雖然藻酸的HPSEC訊號也出現在Peak A，屬於大分子的有機物。但是其多醣類之組成對消毒副產物之貢獻不大，與消毒劑的反應速率也較其他有機物慢。
由混合水樣之HPSEC-OCD之圖譜結果可發現，親水性物質濃度增加會使Peak A面積大幅增加，但是消毒副產物生成之增加幅度較低，使得單位面積增加所貢獻之消毒副產物量較疏水性物質者低。而Peak B面積增加來源是疏水性的腐植酸和黃酸，單位面積的變化對消毒副產物生成有明顯之影響

Disinfection is an important unit in conventional water treatment plant to kill pathogenic microorganisms. Natural organic matter (NOM) exist ubiquitously in drinking water source and have impact on human health due to the formation of disinfection by-products (DBPs) when reacted with disinfectant. Two of the most important DBPs are trihalomethane (THM) and haloacetic (HAA). The main purpose of this study is to understand the relationship between NOM fractions and DBPs formation potential. Four model compounds have been used to simulate the NOM in natural water. Humic acid and fulvic acid are used to represent the hydrophobic organic matters, while BSA (bovine serum albumin) and alginic acid to represent the hydrophilic organic matters. Water quality analysis include global parameters, such as NPDOC, UV254 and SUVA value. And the high performance size exclusion chromatography (HPSEC) with UV/Vis and organic carbon (OC) detectors were used to characterize NOM fractions. The results illustrated that humic acid and fulvic acid had high disinfection by-products formation potential due to their aromatic structures. The high molecular weight BSA also contributed to high THM and HAA formation potential. However, alginic acid, which also has high molecular weight, showed very low reaction rate with chlorine, and low DBPs formation.
Based on HPSEC-OCD chromatograms, the area of peak A (biopolymers) increased proportionally with the concentration of hydrophilic compounds, such as BSA and alginic acid. However, the increase in DBPs concentration per unit increase in organic model compound was low. However, the increase in the area of peak B (humic substances), due to the increase of hydrophobic compounds, such as humic acid and fulvic acid, induced significant DBPs formation. Therefore, it can be concluded that NOM contained in peak B are more significant DBP precursors than those of peak A.

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