轉譯作用由核醣體執行，負責決定基因在細胞中的表現情形。核醣體的組成一直被認為具有高度保留性，然而，近年來的研究卻顯示核醣體蛋白(ribosomal proteins)在不同組織的表現情形有所差異，且會導致特定mRNA優先被轉譯。核醣體蛋白L19 (RPL19)已被報導為前列腺癌和大腸直腸癌的預後指標，且改變RPL19的表現會影響轉譯的精確度及癌細胞的侵略性表現，但RPL19所參與的轉譯調控機制卻仍是未知。在我們先前的研究中，在子宮頸癌細胞Hela內，利用核醣體定位分析技術發現RPL19的差異表現會影響S-phase相關基因的轉譯表現。接著，我們透過預測內部核醣體結合位的資料庫挑出兩個目標基因，並利用雙螢光素酶報導基因分析其IRES活性。我們發現目標基因在Hela中的確具有IRES活性，並且其活性隨著減少RPL19表現而下降。有趣的是，在大腸直腸癌細胞SW480中也呈現相同的結果。因此我們推測在其他癌症的病變過程中或許也存在著相似的RPL19所調控之轉譯機制，於是利用核醣體定位分析技術研究在SW480中基因的表達情形。希望透過這兩種不同的癌症模型，可以進一步釐清RPL19所參與在癌症病變過程中的功能性角色。

The translational regulation mechanism determines the gene expression pattern in cells. Traditionally, the components of ribosome were regard as a highly conserved complex. However, recent studies reveal the expressions of some ribosomal proteins (RPs) vary in different tissues and lead to preferentially translate specific mRNAs. Ribosomal protein L19 (RPL19) has been reported to be a prognostic marker for prostate cancer as well as colorectal cancer. Downregulation of RPL19 would decrease the fidelity of translation and abrogate the aggressive phenotype of cancer. However, the underlying mechanism of RPL19 participating in translation is unclear. In our previous study, we proved reducing the expression of RPL19 affected the expression of S-phase related genes in Hela cells. We selected two of S-phase related genes according to internal ribosome entry site (IRES) prediction database and tested the IRES activity of their 5’UTR by dual reporter assay. We found that these genes indeed carry the IRES activity in Hela cells, and the IRES activity would be decreased with RPL19 knockdown. Intriguingly, the same results also demonstrated in SW480, the colorectal cancer cell line. To further validate the translational mechanism regulated by RPL19 also involved in the progress of other cancers, we explored the change of ribosome binding pattern in SW480 by ribosome profiling. Utilizing the two distinct cancer cell line, we try to further explore the functional role of RPL19 in the progression of cancer.

葉凡綺，利用核醣體定位技術探討核醣體蛋白L19在轉譯調控中的功能，國立成功大學生物科技與產業科學系碩士論文，2018。
鍾秉翰，核醣體蛋白L19結合到週期素D1五端非轉譯區內之核醣體結合區來調控週期素D1蛋白質表現與細胞週期進行，國立成功大學生物資訊與訊息傳遞研究所碩士論文，2012。
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