本研究以串聯連線的多角度光散射雷射光散射(Multi-angle Laser Light Scattering，MALS)、折射率偵測器(Differential Refractometer)及紫外線探測器(UV/Visible Detector)的凝膠滲透層析(Gel Permeation Chromatography)探討在不同十二烷基硫酸鈉(Sodium dodecyl Sulfate， SDS)濃度下，聚(N-乙烯甲醯胺)(PNVF)與SDS之間的交互作用，及PNVF分子鏈上吸附的SDS對PNVF構形的影響。從多成份系統的光散射原理可計算PNVF與SDS形成的複合物分子量、z-平均環動半徑(z-average radius of gyration，Rz)，和複合物中的PNVF分子量，以及利用Hummel-Dreyer法計算SDS在PNVF分子鏈上的結合比率(binding ratio)。在SDS濃度介於cac與cmc之間，從PNVF/SDS複合物中的PNVF分子量可以發現，PNVF分子量隨著SDS濃度增加而增加，這顯示在PNVF/SDS系統中，PNVF濃度低於臨界濃度c*仍有分子鏈間的交互作用。

The novel method to characterize the polymer-surfactant interaction is using gel permeation chromatography with online multi-angle laser light scattering, differential refractometer, and UV/Visible detector. In this study, the interaction between poly(N-vinylformamide) (PNVF) and sodium dodecyl sulfate (SDS) under several SDS concentrations has been investigated by using this technique to confirm the influence of SDS bound into the PNVF/SDS complex on the molecular shapes of PNVF. The application of both Hummel-Dreyer method and multi-component light scattering principle provides much useful information concerning the determinations of the binding ratio (the amount of SDS bound to PNVF), the z-average radius of gyration of PNVF/SDS complexes (Rz), and the molecular mass of PNVF/SDS complexes (Mw,PS) and the PNVF itself within the PNVF/SDS complexes (Mw,P). From the measurements of the molecular mass of PNVF in the PNVF/SDS complexes at different SDS concentrations, the results show that the Mw,P increasing with increasing SDS concentrations between cac and cmc. It indicates that a significant intermolecular association between cac and cmc in PNVF/SDS system, even when the PNVF concentration below the critical overlap concentration (c*).

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