本研究開發出具有綠色螢光以及MRI顯影特性的矽酸釓螢光奈米殼(Gd silicate:FITC nanoshell)，其粒徑大小約為50~60 nm，並將矽酸釓螢光奈米殼包覆ㄧ層20 nm中孔洞二氧化矽層(Gd silicate:FITC@mSiO2)，提高其材料表面積達到高裝載或修飾藥物的能力。藉由螯合釓離子在其二氧化矽孔洞內，增強了其核磁共振造影顯影的能力，其材料除了表現了優異的T1顯影劑的能力，更發現我們的材料具有磁場敏感性，在較高的磁場能展現出強的T2顯影能力，在高磁場7T底下，其r2值相較於磁場3T下高出了4到5倍。進而開發螯合釓離子的中孔洞二氧化矽包覆矽酸釓螢光奈米殼(Gd3+ chelated Gd silicate:FITC@mSiO2)新型T1和T2雙顯影劑。
另一方面藉由修飾MHI-148在中孔洞二氧化矽包覆矽酸釓螢光奈米殼表面，結合MHI-148有機分子，癌細胞會藉由有機陰離子傳輸縮氨酸管道(organic anion transporting peptides)將其攝入，使之具有近紅外光放光和標定癌細胞能力，達到同時具有核磁共振造影顯影和可穿透表皮組織近紅外光螢光的能力，這種利用雙顯影的標定功能，將能更準確的定位偵測體內癌細胞腫瘤位置。

This study aim to develop new nanocomposite of Gd silicate:FITC nanoshell (50~60 nm) with fluorescence integration and MR imaging ability. Gd silicate:FITC nanoshell served as a core and further encapsulated within 20 nm mesoporous silica shell to fabricate Gd silicate:FITC@mSiO2, this modification increase the particle’s surface area and loading ability. Gd3+ was used to chelate the interior channels of mesoporous silica, resulting in Gd3+ chelated Gd silicate : FITC@mSiO2 nanoparticle. The existence of Gd3+ within the mesoporous silica channel, can promote the transverse r1 relaxivity.Most interestingly, the Gd-containing silicates have an advantage in serving as negative contrast agents with effective T2-shortening characteristics in strong fields. The transverse relaxivity (r2) values enlarged in 4-5 times as field increased from 3T to 7T. In animal MR imaging
In addition, MHI-148 was conjugated with Gd silicate:FITC @mSiO2 nanoparticle, which can modify the particle with near-infrared(NIR) radiation and targeting cancer cells ability, The cancer-specific uptake and retention of MHI-148 is likely to be mediated by organic anion transporting peptides (OATPs). MHI-148 conjugated Gd silicate:FITC@mSiO2 nanoparticles were fabricated to display dual modalities expressing MR and optical imaging. Future application of NIR fluorescent and MR images in the clinical used could lead to important progress in the management of cancer patients on an individual basis.

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