在南臺灣，埃及斑蚊是傳播登革熱病毒的主要媒介，此疾病會造成感染者關節疼痛、發高燒等症狀，嚴重時更會造成死亡，故國際間常用的昆蟲不孕技術被我國納入考量。當使用廣泛存在於節肢動物體的沃爾巴克氏菌感染埃及斑蚊時，可延伸出兩種生物防治策略，第一種是釋放大量攜帶沃爾巴克氏菌的雄蚊與野生雌蚊交配，攜帶沃爾巴克氏菌的雄蚊精子會影響受精卵發育，使得野生雌蚊交配後產下的卵無法孵化，抑制野生族群的數量。第二種是同時釋放攜帶沃爾巴克氏菌的雌、雄蚊，利用沃爾巴克氏菌能母系垂直傳染及營救受精卵的特性，放大攜帶沃爾巴克氏菌的族群，進而取代野生埃及斑蚊族群。再者，有文獻指出沃爾巴克氏菌能抑制埃及斑蚊體內複製登革熱病毒的能力，達到難以傳染人類登革熱的病毒量，藉此壓制登革熱病例數。本篇研究針對族群取代法在實驗室進行測試，同時本研究第二部分利用遙控無人履帶車系統(Unmanned Ground Vehicle Systems-UGVs) 探勘高雄市歷年好發登革熱的行政區，對病媒蚊生態進行追蹤及投藥，並透過成蚊密度監測結果來分析UGVs的影響力，若未來UGVs能夠掛載小蚊籠進入陽性水溝投放攜帶沃爾巴克氏菌的埃及斑蚊或許能夠達成「以蚊尋蚊、以蚊滅蚊」的構想。

Aedes aegypti is the vector of dengue in southern Taiwan. Dengue can cause such as joint pain and high fever. Therefore, commonly used sterile insect technology in the world has been taken into consideration in Taiwan. When symbiotic bacterium – Wolbachia infects Aedes aegypti, two biological control strategies will be established. The first is population suppression, which releases a lot of Wolbachia - carrying males to mate with wild type females, the sperm from Wolbachia - carrying males will make the eggs produce by wild type female cannot hatch, that can suppress the density of wild type group. The second strategy is population replacement, Wolbachia can vertically infect next generation by females and rescue fertilized eggs. As release a lot of Wolbachia - carrying males and females, the population will keeping amplify and replace wild type groups someday. There are some report shows Wolbachia can reduce the replication ability of dengue virus in Aedes aegypti, making the titers of dengue virus cannot be spread between humanity. This study also used UGVs (Unmanned Ground Vehicle Systems) to inspect the ditches of five administrative districts that dengue fever has been prevalent over the years in Kaohsiung. After the UGVs inspection and immediately administer the drug for positive ditches, the density of vector mosquitoes decreased significantly next week. If we use UGVs to inspect the positive ditches and release Wolbachia - carrying Aedes aegypti. Maybe we can achieve the concept of finding mosquitoes with mosquitoes and eliminating mosquitoes with mosquitoes.

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