在此論文中，設計並發展一個微型的非線性光學掃描探頭，結合一微型馬達驅動以獲得3D掃描影像。實驗以GriffinTM Ti:sapphire飛秒雷射震盪器作雷射光源，透過一條雙包覆層光纖中的單模纖芯傳送電射脈衝，多模內包覆層收集非線性的光學訊號。其中利用三片消色差透鏡組降低長波常激發光與短波長信號之間的色差，藉以提昇訊號的收集效率。另一對雙稜鏡組產生負色散，藉以補償整個系統，尤其是光纖部分所造成之正色散，提高非線性光學激發的效率。
壓電管與光纖整合後，以適當的電壓作驅動，利用螺旋掃描方式，產生一個在空間上填滿的圖形，並透過光電倍增管擷取二維非線性光學影像。螺旋式掃描的視野範圍直徑是60 μm，幀速率是5 Hz，光纖掃描的共震頻是855 Hz，影像像素為273*273。在此論文中，二維的非線性光學掃描管與微型的壓電馬達整合在一起，使得二維掃描管能在軸向上移動，達到三維影像掃描之目的。本實驗製作的三維非線性光學掃描探頭，寬度為6 mm而長度為6.4 cm。

In this thesis, a three-dimensional (3D) nonlinear optical endoscope with the depth scanning capability has been developed. It includes a developed 2D fiber-optic scanning endoscope and combines with a micro-motor for axial scanning. Therefore, it provides 3D nonlinear optical images such as two-photon excitation fluorescence and second harmonic generation. The developed 2D scanning probe includes a double clad fiber to develop the laser pulses and collect nonlinear optical signal, a spiral fiber scanner forced by a piezoelectric tube, an assembly of three miniature achromatic lenses to reduce the focal shift, and a micro-motor and some z-axis components for achieving the depth scanning. A pair of double prisms can produce the negative group velocity dispersion mainly from the fiber to compensate the dispersion.
In the 2D scanning tube, a spiral scanning pattern is utilized to drive the fiber cantilever to obtain 2D nonlinear optical images. The field of view is approximately 60 μm in diameter, the pixels are 273*273, the frame rate is 5 Hz, and the resonant frequency is 855 Hz. The 2D scanning tube is housed with a micro-motor for z-axis motion. Furthermore, all components are integrated into a 3D nonlinear optical endoscope. The diameter of the probe is 6 mm and the length is 6.4 cm. The micro-motor achieves axial scanning. Finally, the 3D nonlinear optical images are obtained by a compact, feasible, and fiber-based endoscope.

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