隨著科技及文明發展，石化能源對人類日益重要。近年來無論在國內外均發生嚴重的油汙染事件，如儲油槽洩漏、油輪擱淺等事件。這些事件不但會在當時造成嚴重污染環境生態，且在多年後的今日，受污染的區域仍無法回復往日的生機。利用生物進行土壤生物復育成為現今復育技術的趨勢因費用低廉及低技術，但生物復育需長時間操作為此技術最大的缺點。本研究分為兩大主題，其一為以土壤管柱模擬現地生物復育，其二為以小型土壤反應槽模擬現址生物復育(土耕法)，藉由添加菌劑及生物界面活性劑，期望有效的縮短復育時間。探討柴油去除率、微生物菌量變化、動力推導(K)及以分子生物學角度切入微生物生態的變遷。
首先，探討土壤管柱不同復育方式對去除率的影響，分別以營養促進(Nutrient Enhanced,NE)、生物刺激(Biostiumlation.BS)生物強化(Bioaugmentation,BA)，及控制組(Sterile Soil,SS)操作。土壤管柱操作330天後，柴油殘留濃度分別為3026、4105、1851及6506 mgTPH-d/kg dry soil，9柴油降解率分別為66.6﹪、44.9﹪、80.2﹪及23.6﹪，以生物強化的效率最佳。以分子生物技術偵測不同時期土壤管柱內的微生物生態變化，90天操作後發現BA管柱內額外添加優勢柴油菌消失，並偵測到數株具有碳氫分解能力菌種。
其次，探討添加優勢菌群於土壤反應槽內，對柴油去除率的影響，以不同植菌量及添加生物界面活性劑作為實驗因子。各組柴油去除率均界於80%~90%，遠高於控制組去除率(50%)。批次實驗的柴油數據模板(pattern)符合一階動力，經一階動力迴歸求得柴油降解常數(k)，以添加生物界面活性劑於高植菌的組別0.027day-1最快。添加菌劑及生物界面活性劑去除50%只需49天，但控制組需經90天以上時間。綜合來說添加菌劑可有效提高柴油降解率速率，能縮短二倍以上復育時間。

With the technology generation and human culture developing, petroleum energy is more important in human life. Recently, petroleum pollution was became worldwide problem, taking example for tank spill etc. This accident was result in danger of environmental ecology and the pollution is not easy to reconvert to original situation after many years. Bioremediation became the worldwide trade because the hydrocarbon bioremediation is more economic and low technology than other remediation. Because bio reaction is slowly and bioremediation is a long-term operation is a serious disadvantage in this technology. There is two major topics in this study. First, using soil column to simulate the in-situ bioremediation. Next using lab-scale soil microcosm to simulate the on-site bioremediation (Land farming). The bioremediation performance was evaluated by diesel removal efficiency, the trade of microrganism growth and kinetic analyses, and monitoring the changing of microrganism dynamics by molecular technology.
First, evaluating the effect of removal in soil column by different bioremediation methods which is Nutrient Enhaced(NE), Biostiulation (BS),Bioaugmentation (BA) and Control(Sterile Soil,SS).The diesel residual concentration is 3026 ,4105 ,1851 and 6506 mgTPH-d/kg dry soil and biodegradation efficiency is 66.6%, 44.9%,80.2% and 23.6% in soil column after 330 day operation. According to residual concentration result, the best performance is BA soil column. The additional dominant diesel-degradation bacteria disappeared after 90 days operation in BA soil column and several the potential diesel-degradation bacteria from reference observed in soil column by molecular biotechnology monitoring.
Next, evaluating the effect of removal in Lab-scale soil microcosm batch test by different consortia. The experimental factor is different bioaugmentation density and upgrading bioavailability by adding biosurfactant. Comparison diesel removal with Control, the removal efficiency of experimental microcosm is 80% to 90% is higher than removal efficiency of control is 50%.The diesel degradation patterns fit first-order kinetic. Comparison the diesel degradation constant by first-order kinetic, the adding biosurfactant in high bioaugmentation density is fast. The bioaugemntation by adding consortia and surfactant can raise the degradation rate and shorten the time of bioremediation over twice time.

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