本研究採用兩步驟加成法合成出三種不同比例的聚胺基甲酸酯(polyurethane)，透過碘辛烷(1-iodooctane)的作用，可以將聚胺基甲酸酯上啶(pyridine)的三級胺四級化，使得四級化聚胺基甲酸酯帶有正電性，具有破壞細菌細胞膜表面的效果，並進一步做緩衝液流動場細菌循環吸附測試，探討在流動的狀態下抑制細菌吸附的效果。經由元素分析(EA)、傅利葉紅外線光譜(FT-IR)、核磁共振分析(NMR)結果，確認高分子的結構與組成。微差掃瞄熱卡儀(DSC)與熱重分析儀(TGA)分析熱性質，得知聚胺基甲酸酯會因為四級化，造成氫鍵破壞，熱穩定性下降，熱性質也下降。此外，四級化聚胺基甲酸酯帶有四級化的啶官能機團(quaternized pyridine group)，使得吸水能力與親水性隨著四級化的程度增加而增加。
　　緩衝液流動場細菌循環吸附測試中，未四級化(電中性)聚胺基甲酸酯吸附細菌量探討，發現隨著親水性的遞增，細菌吸附量明顯遞減。四級化(正電性) 聚胺基甲酸酯，預期利用帶有正電性的特性，使得細胞吸附上去後，細胞膜遭到破壞而死亡。但實驗結果顯示，反而因為帶有正電性，導致表面帶有負電荷的細菌大量吸附，吸附量因此超越未四級化(電中性)聚胺基甲酸酯。

　　Three families of non-release quaternized polyurethane were synthesized by different molar ratio of MDI, PTMO and N, N-bis(2-hydroxyethyl)isonicotinamide (BIN). BIN was used as the chain extender in a series of polyurethane block copolymer. The structure of pyridine on BIN could be quaternized by 1-iodooctane and formed cationic polyurethanes. The structure of cationic polyurethanes was proven by the characterization results of nuclear magnetic resonance (NMR), element analysis (EA) and fourier transform infrared spectroscopy (FTIR). Surface hydrophilicity in water and water absorption ratio were both increased with the quaternization ratio because of incorporation of ionic functionality. The antibacterial capability of cationic polyurethanes was assessed by 24 hours continuous in vitro E. coli perfusion test. The polyurethane with quaternized pyridine group had much more bacterial adhered than electro neutral polyurethane and PE. This should result from the electrostatic interaction between the positive charge along the quaternized pyridine groups and the negative charge associated with bacterial cell membrane. This study shows that the quaternized polyurethane exerts a strong adhesive force on E. coli, even quaternized group possess biocide ability.

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