現地生物整治時擠注之水溶性基質及菌劑常侷限在傳統注入井的井口附近，未能有效分布在含水層中。為解決以上工程瓶頸，本計劃創新研發串聯式水平多孔隙管擠注系統擠注水溶性基質及菌劑，提升擠注水溶性基質及菌劑在含水層中之有效分布。水溶性基質的擠注以氚水為代表，菌劑的擠注以大腸桿菌（E. coli）水溶液為代表。根據氚水及大腸桿菌水溶液現地擠注結果可知串聯式水平多孔隙管擠注系統能有效提升水溶性基質及菌劑的擠注有效範圍達12.6公尺以上。現地串聯式水平多孔隙管水溶性基質擠注效率為102%，菌劑擠注效率為44%，多孔隙管的過濾機制使得菌劑擠注效率低於水溶性基質擠注效率。

The efficiency of in-situ bioremediation observed in the field is often lower than that observed in laboratories. One of the major reasons is due to the contact efficiency for aqueous solution of substrates and bacteria in the field are lower than that obtained in laboratories. The objective of this study is to apply a horizontal-porous-tube injection system to improve the contact efficiency for aqueous solution of substrates and bacteria in the field. Tritium solution was used to represent the aqueous substrates, and Escheriohia coli （E. coli） was used to represent the bacteria. The recovery was 102 % and 44 % for tritium and E. coli, respectively. The filtration mechanism could be responsible for the lower recovery of E. coli much lower than tritium. Pilot injection tests demonstrated that the horizontal-porous- tubes injection system could effectively improve the contact efficiency for aqueous solution of substrates and bacteria up to 12.6 m from the injection point.

蔡文田、邱伸彥，“蒸氣脫脂用含氯溶劑之特性管制和污染預防”，工業污染防治，第41期，第145-160頁，1992。
行政院環境保護署，水中菌落數檢測方法-混合稀釋法，NIEA E203.54B, 2005。
陳漢衛，“生物阻塞之實驗研究”，碩士論文，國立成功大學資源工程研究所，台南，2007。
Duba, A.G., K.J. Jackson, M.C. Jovanovich, R.B. Knapp, and R.T. Taylor, TCE remediation using in situ, resting-state bioaugmentation, Environmental Science and Technology, v. 30, n. 6, pp. 1982-1989, 1996.
Hopkins, G. D., Semprini, L., and McCarty, P. L., Microcosm and In Situ Field Studies of Enhanced Biotransformation of Trichloroethlene by Phenol-Utilizing Microorganisms, Appl. Environ. Microbiol., v. 59, n. 7, pp. 2277-2285, 1993.
Hopkins, G. D., and McCarty, P. L., Field Evaluation of in Situ Aerobic Cometabolism of Trichloroethylene and Three Dichloroethlene Isomers Using Phenol and Toluene as the Primary Substrates, Environ. Sci. Technol., v. 29, n. 6, pp. 1628-1637, 1995.
Kuo, M. C. T., Chen, C. M., Lin, C. H., Fang, H. C. and Lee, C. H., Surveys of Volatile Organic Compounds in Soil and Groundwater at Industrial Sites in Taiwan, Bulletin of Environmental Contamination & Toxicology, v. 65, n. 5, pp. 654-659, 2000.
Kuo MC Tom, Liang KF, Han YL, Fan KC. Pilot studies for in-situ aerobic cometabolism of trichloroethylene using toluene-vapor as the primary substrate. Water Research, v. 38, n. 19: 4125-4134, 2004.
Lovelace, K. A., Evaluating the Technical Impracticability of Groundwater Cleanup, 1997 International Conference on Groundwater Quality Protection, Taipei, pp. 165-179（1997）.
McCarty, P. L., Goltz, M. N., Hopkins, G. D., Dolan, M. E., Allan, J. P., Kawakami, B. T., and Carrothers, T. J., Full-Scale Evalution of In Situ Cometabolic Degradation of Trichloroethylene in Groundwater through Toluene Injection, Environ. Sci. Technol., v. 32, n. 1, pp. 88-100, 1998.
Roberts, P.V., G.D. Hopkins, D.M. Mackay, and L. Semprini, A field evaluation of in-situ biodegradation of chlorinated ethenes: Part 1, methodology and field characterization, Ground Water, v. 28, n. 4, pp. 591-604, 1990.
Semprini, L., P.V. Roberts, G.D. Hopkins, and P.L. McCarty, A field evaluation of in-situ biodegradation of chlorinated ethenes: Part 2, results of biostimulation and biotransformation experiments, Ground Water, v. 28, n. 5, pp. 715-727, 1990.
Semprini, L., G.D. Hopkins, P.V. Roberts, D. Grbic-Galic, and P.L. McCarty, A field evaluation of in-situ biodegradation of chlorinated ethenes: Part 3, studies of competitive inhibition, Ground Water, v. 29, n. 2, pp. 239-250, 1991.
Semprini, L. and P.L. McCarty, Comparison between model simulations and field results for in-situ biorestoration of chlorinated alphatics. Part 1. Biostimulation of methanotrophic bacteria, Ground Water, v. 29, n. 3, pp. 365-374, 1991
Simpkin, T. J., Carothers, G., Hoffman, R. W., Elder, R. and Collver, B. F., The Influence of Temperature on Bioventing In: In Situ Aeration: Air Sparging, Bioventing, and Related Remediation Process, Hinchee, R. E., Miller, R. N. and Johnson, P. C. （Eds.）, Battelle press, Columbus, pp. 315-322, 1995.