本研究以大型藻類為對象，於五溝水樣點進行藻類採集、水文環境因子收集及水質分析，透過統計分析釐清上湧及下滲區水文環境因子之差異，並探討伏流水其上湧及下滲特性對大型藻類分布是否具有顯著影響。
研究於2020年1月到4月於五溝水當地挑選四個樣點，分別為一號水門、廣泉堂、社區公園及屋背溝進行藻類採樣及水質分析，藻類研究以穿越線調查藻類覆蓋度，並採樣以鏡檢方式分辨出藻種，最終以藻類覆蓋率及藻屬百分比計算得出藻屬豐富度。五溝水樣點上湧及下滲區參考前人研究，於樣點進行微測壓管打管作業，判定上湧及下滲結果並記錄，隨後進一步測量垂直水力梯度，最後與藻種及豐富度進行比較分析，了解其大型藻種類及豐富度是否與上湧及下滲區之水文環境因子具有顯著影響。
藻類採樣結果發現五溝水主要大型藻類為水綿屬(Spirogyra sp.) 與板藻屬(Mougeotia sp.)，皆屬於綠藻門。依垂直水力梯度分級結果與藻類分布比例進行分析，檢定結果發現水溫、pH、葉綠素a及水綿屬豐富度有顯著差異，在導電度、溶氧、流速、水深、板藻屬豐富度無顯著性差異。依穿越線調查大型藻覆蓋率與藻比例進行藻屬豐富度計算，分級結果顯示水綿屬高豐富度多在廣泉堂，而板藻屬高豐富度多分布在社區公園。依垂直水力梯度比較藻屬豐富度分析，板藻屬低豐富度檢定結果僅溶氧有顯著差異，其餘環境因子無顯著差異。

The study tried to figure out how the upwelling and downwelling of hyporheic water affects periphyton. Sampling sites were examined by collecting periphyton, surveying environmental factors, and investigating hyporheic water in the Wu Gou Shui. Data was from January to April 2020. Four sections, No. 1 Water Gate, Guang Quan Hall, Community Park and Wu Bei Gou, were sampled. The macroalgae coverage were investigated by transect lines in the river, then they were observed under the microscope to distinguish the algae species and calculate abundances. The vertical hydraulic gradient were surveyed by the mini piezometers. The water quality data in hyporheic zone were recorded (pH, conductive, dissolved oxygen, and water temperature). The results showed that the main macroalgae in Wu Gou Shui were Spirogyra sp. and Mougeotia sp. The vertical hydraulic gradient and the macroalgae collected sites were different with water quality (water temperature, pH, Chlorophyll a, and Spirogyra sp. abundances). The macroalgae abundances showed that the high abundance of Spirogyra sp.was mostly in Guang Quan Hall, and Mougeotia sp. was mostly distributed in the community park. The vertical hydraulic gradient and the macroalgae abundances were only different with dissolved oxygen, and no significant differences in other environmental factors.

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