本研究成功開發出具有高線性度、高靈敏度及穩定度之手持式快速檢測系統，利用免疫反應呈色結果作為檢測標的，使免疫呈色反應偵測於定量分析上更加精確。本研究開發之手持式快速檢測系統包含硬體及軟體兩部分，硬體方面設計出一台呈色判讀儀主體，裝載Raspberry Pi 2 Model B開發板、Universal Serial Bus (USB)攝影機、LED光源、LCD觸控式液晶顯示器、整合式電路板、RTC模組(Real-time clock)、導光板、反射片及光學暗房，並製作出多功能載台，正反面分別為檢測卡匣放置區與快速響應矩陣碼黏貼區。軟體部分使用Python整合模組Zbar、OpenCV、NumPy及Matplotlib撰寫出影像處理程式。由實驗結果可發現，本研究所開發之手持式快速檢測系統對幽門螺旋桿菌之檢測試劑量測下，正確讀取使用者輸入之Control、Test線(C、T線)位置，並建立出高線性度之濃度與灰階值校正曲線，作為量化分析依據，該曲線之R square值可達0.9921，在幽門螺旋桿菌濃度極限偵測下，可量測最低濃度為2500 CFU/mL，成功地突破傳統閾值判定，並且在重複實驗下仍可保持良好的精準度(變異係數<20 %)。

This thesis presents a highly sensitive and stable handheld rapid test system for Helicobacter pylori (HPY). The inspection is based on immunochromatographic assay which is widely applied to Helicobacter pylori detection.Combined with the immunochromatographic strip, the handheld rapid test system is able to detect and calculate HPY concentration. The system is composed of hardware and software parts, and the layout of rapid reader was designed by SolidWorks. The analyzing programs including a main program, QR code and image processing were compiled by Python. After verification of each parts of the system, we found that the rapid test system successfully detected Helicobacter pylori reagent from 2500 CFU/mL to 8500 CFU/mL.

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