癲癇是一種非常常見的神經系統疾病，世界上約有不到百分之一的人口患有這種疾病。感謝醫藥技術的發達，至今已存在多種的治療方法，像是抗癲癇藥物或是癲癇手術，可以根據個別的症狀對症下藥。但至今仍有大約四分之一的癲癇患者是沒有辦法透過現存的醫療技術有效治療或是減輕他們的症狀的。腦部的不正常放電是癲癇發生的主因。因此，腦電波自然成為觀察、偵測癲癇，甚至更進一步開發治療方法的最佳工具。而多項以腦電圖為基礎開發的閉迴路式腦部電刺激系統已被提出且付諸實行，目前NeuroPace的RNS還在美國食品要管理局許可下臨床實驗。而Medtronic的the Intercept™ Epilepsy Control System則於2012年五月結束試驗，在兩年內，有過半的受試者有超過50%的癲癇頻率改善，另有14名受試者6個月癲癇發作。在這篇論文中，除了討論閉迴路式的電刺激抑制系統於實驗大鼠身上的成效外，我們還觀察長時間使用所造成的影響，採用的實驗動物為天生便患有失神性癲癇的Long-Evans大鼠。在整個實驗過程中，我們將會仔細的紀錄下牠的生理現象，像是施行電刺激抑制部位的阻抗值、腦波訊號、體重變化。透過仔細觀察各星期間棘狀放電波形的變化，並將其差異量化成各式的描述用指標來表達實驗其間造成的差距到底有多大。關於電刺激抑制部位的阻抗值兩隻實驗鼠中，一隻無明顯變化情況，另一隻則較有不隨時間的波動現象。而癲癇波形的最大尖峰能量亦有變化產生，一隻出現上昇的現象，另一隻則呈現下降。但兩者的癲癇出現的規律都傾向於以區域高密集的癲癇群的方式出現。

Epilepsy, one of the most general neurological symptoms, occurs among 0.6-0.8% of the world’s population. Nowadays, thanks to medical advances, already exists certain therapies, such as anti-seizure medication, respective surgical operation, that can be taken according to individual circumstances to ease it, but still for about a quarter of the patients not yet the sufficient treatment is available. Abnormal discharge in brain is what causing epilepsy to happen. Due to this fact, electroencephalogram (EEG) is the perfect tool to observe, measure, detect and, furthermore, develop treatment. Brain stimulation with close loop control had already been proposed and put in practical; NeuroPace’s RNS is in U.S. FDA. clinical trial. And another clinical trial of Medtronic’s InterceptTM finished at May 2012, with half of patients’ seizure reduced at least 50%, and 14 patients were seizure-free for at least 6 months in 2 years. The model animal in this experiment is Long-Evans rats with absence seizure. Beside of reporting the efficiency, we observed the long term effects of applying the brain stimulation with close loop control. We carefully recorded the EEG signal during the whole experiment, measured impedance of the brain tissue which had been stimulated and weight weekly, studied the spike-wave-discharge (SWD) form EEG records and then calculated several indices to describe the phenomenon we discovered. One of the subjects showed no major fluctuations in impedance, the other one did. The seizure wave form and the pattern of occurrence changed. The changed of wave form wasn’t consistent over our two subjects, one tend to increased, the other opposite, but both of the pattern of occurrence started to a tendency of occurring in group.

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