本研究結合嗅覺及儀器方法分析水中放線菌所產生臭味物質及其受氧化劑作用之影響，前者以嗅覺氣相層析儀(Sensory GC)為主來分析臭味物質之氣味及強度，後者則以固相微萃取法(Solid-phase microextraction, SPME)配合氣相層析質譜儀(GC/MS)分析臭味物質之種類及濃度。研究中發現Streptomyces caelestic及Streptomyces malaysiensi會產生果臭味的2-Methyl-butanoic acid與3-Methyl-butanoic acid；造成香蕉臭味的6-Methyl-2-heptanone；造成花香味的2-Ethyl-1-hexanol、gamma-Cadinene與Linalool；造成藥草味的Menthol；造成土霉味的MIB與Geosmin；以及果味的Heptanal、Heptanol。其中2-Methyl-butanoic acid之閾值為100μg/L；3-Methyl-butanoic acid之閾值為7.7μg/L；6-Methyl-2-heptanone之閾值為300ng/L；2-Ethyl-1-hexanol之閾值為4μg/L；Linalool之閾值為62.5ng/L；Menthol之閾值為12.5μg/L。放線菌產生之臭味物質大部分存在於細胞外(extracellular)，土霉味物質存在於細胞外的比例約為50%~70%。

　　三種常用之氧化劑，臭氧、氯及高錳酸鉀，針對放線菌存在之水樣進行土霉味物質之氧化，發現去除率只有約30%，顯示放線菌存在時，氧化土霉味物質之效果不佳。

　　臭氧氧化放線菌存在之水樣，可能會導致Heptanal、Heptanol與6-Methyl-2-heptanone的增加。Linalool、Menthol、2-Methyl-butanoic acid及gamma-Cadinene則會因為氧化劑的添加，而有氧化去除的現象。

　Drinking water quality in southern Taiwan receives complains from customers for a long time for its unpleasant odors. Earthy/musty and fishy odors were the two major odor groups in many drinking water sources. Two Streptomyces metabolites, 2-MIB and geosmin, are known to be the predominated musty and earthy odor compounds in most of the surface drinking water sources in Taiwan. Unlike that for the musty and earthy odor, the chemicals responsible for other odors are not identified. In this study, the responsible chemicals for the odor generated by Streptomyces caelestis and Streptomyces malaysiensis were investigated using both sensory and instrumental methods. A solid phase micro-extraction (SPME) technique was employed to concentrate the odorants from the incubated Streptomyces samples. A gas chromatograph (GC) coupled with a mass spectrometer detector (MSD) and an olfactory port in parallel was used to determine both the chemicals and their corresponding odor types trapped in the SPME fibers.

　Nine chemicals, responsible for sweat odor (2-methyl-butanoic acid and 3-methyl-butanoic acid), floral odor (linalool, 2-ethyl-1-hexanol and gamma-cadinene), herbal odor (menthol) and fruity odor (6-methyl-2-heptanone, heptanal and heptanol) were identified. The odor thresholds of 2-Methyl-butanoic acid, 3-methyl-butanoic acid, 6-methyl-2-heptanone, linalool, menthol and 2-ethyl-1-hexanol are 100 g/L, 7.7g/L, 300g/L, 62.5ng/L, 12.5g/L and 4.0g/L, respectively. These odorants were most present within the cells of Streptomyces, and the extra- to intra-cellular ratios of MIB and geosmin were approximately at 50-70 %.

　Three oxidants, sodium hypochlorite, potassium permanganate, and ozone, were tested for the removal of 2-MIB and geosmin with presence of Streptomyces caelestis or Streptomyces malaysiensis. Only about 30% of removal efficiency were observed for all the three tested oxidants. Besides, in applying ozone into the Streptomyces-laden water samples, the concentration of heptanal, heptanol, and 6-methyl-2-heptanone increased due to the release from cells. On the other hand, linalool, menthol, 2-methyl-butanoic acid and gamma-cadinene were destructed by the oxidants.

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