毛細管電泳(Capillary Zone Electrophoresis，簡稱CZE)為近年來迅速發展的一種分析方法。而在毛細管電泳中，電雙層效應造成靠近管道附近的不同的離子分布密度，此現象吸引與壁面相反電荷的異性離子(Counter-ions)於壁面附近聚集，而同性離子(Co-ions)則會受到排斥的力量而遠離壁面。
本研究將探討不同德拜厚度對於毛細管電泳晶片中帶電溶質離子的遷移現象。因注入管道與分離管道交會處為一圓弧區，為求在圓弧區能有較精準的數值結果，故使用有限元素法(Finite Element Method)來計算並採用粒子模擬法(Particle-In-Cell Method)以瞭解微管道內不同帶電性質溶質離子之運動現象。
由結果中亦發現在較小的德拜厚度下，電雙層影響範圍較小，因此造成離子不論在注入管道或分離管道中，受壁面吸引或排斥的效應不明顯。流場兩側受到的擠壓較小，流線能較往分離管道中兩側擴展，將更多離子帶進分離管道中。負離子在圓弧區無聚集現象，使得訊號輸出時有較好的分析趨勢；正離子在分離過程中，吸附在壁面上的比例較少。
因此可得知在較小的德拜厚度之下，能得到更多的樣品資訊作為分析。

Capillary Zone Electrophoresis (CZE) is a kind of analysis method that develops rapidly in recent years. The effects of EDL cause different ions distribution density near the wall of CZE. Furthermore, the counter-ions will be pulled toward the microchannel wall of opposite charge and the co-ions will be pushed out of the EDL of the same charge.
Therefore, the effect of different Debye lengths on charged ions migration in CZE will be investigated in this study. In order to get the more accurate result for circular-arc in the intersection of injection channel and separation channel, this study uses Finite Element Method (FEM) in the computation and employs the Particle-In-Cell (PIC) method to investigate the migration of negatively and positively charged ions in CZE chip.
The results show that smaller Debye length has smaller effective zone and causes streamlines to expand widely, so many charged ions are pushed in separation channel. Because there is no cluster of negative-charged ions at arc-corner and smaller decreases of positive-charged ions during separation process, we can get more data output for analysis and more precise results.

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